Western blot abreview for Anti-PDP1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (MCF7 mitochondrial lysate and MCF7 whole cell lysa)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
MCF7 mitochondrial lysate and MCF7 whole cell lysa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 1%BSA
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP: sc-2301
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
20 second(s)
Bands
Specific: ~65 kDa

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Submitted Dec 07 2015

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