Product nameAnti-PDP2 antibody [MS41A 3C3BC10]
See all PDP2 primary antibodies
DescriptionMouse monoclonal [MS41A 3C3BC10] to PDP2
Specificityab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Full length protein. This information is considered to be commercially sensitive.
- Recombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesNear homogeneity as judged by SDS-PAGE. ab133982 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Clone numberMS41A 3C3BC10
Light chain typekappa
Our Abpromise guarantee covers the use of ab133982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Note: This antibody works optimally in 5% Milk for all blocking and antibody steps.|
FunctionCatalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
Sequence similaritiesBelongs to the PP2C family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 antibody
- KIAA1348 antibody
- mitochondrial antibody
All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Recombinant human PDP2 protein (ab110358) at 0.01 µg
Lane 3 :
Recombinant Human PDP1/PDP protein (His tag) (ab110357) at 0.01 µg
Lane 4 :
Recombinant human PDK1 protein (ab110359) at 0.01 µg
Lane 5 : HHH (Human heart homogenate) at 20 µg
Lane 6 : HLH (Human liver homogenate) at 20 µg
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 5 minutes
Predicted mature band size: 52 kDa
Observed band size: 52 kDa
Note: The antibody recognizes both the mature (52 kDa) and the immature (60 kDa) versions of PDP2 protein. The immunogen was mature PDP2 protein, lacking the first 66 amino acids which are used as a transit peptide for mitochondrial targeting. The antibody produces a band against the endogenous protein at the mature weight in tissue homogenates, but not in cancer cells where the protein may fail to mature.
This product has been referenced in:
- Ryan ZC et al. 1a,25-dihydroxyvitamin D3mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells. Biochem Biophys Res Commun 496:746-752 (2018). WB . Read more (PubMed: 29366785) »
- Ryan ZC et al. 1a,25-Dihydroxyvitamin D3 Regulates Mitochondrial Oxygen Consumption and Dynamics in Human Skeletal Muscle Cells. J Biol Chem 291:1514-28 (2016). Read more (PubMed: 26601949) »