Overview

  • Product name

    Anti-PDP2 antibody [MS41A 3C3BC10]
    See all PDP2 primary antibodies
  • Description

    Mouse monoclonal [MS41A 3C3BC10] to PDP2
  • Host species

    Mouse
  • Specificity

    ab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Recombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab133982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Note: This antibody works optimally in 5% Milk for all blocking and antibody steps.

Target

  • Function

    Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Sequence similarities

    Belongs to the PP2C family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 antibody
    • KIAA1348 antibody
    • mitochondrial antibody
    • PDP 2 antibody
    • Pdp2 antibody
    • PDP2_HUMAN antibody
    • PDPC 2 antibody
    • PPM2B antibody
    • PPM2C2 antibody
    • Protein phosphatase 2C magnesium dependent catalytic subunit 2 antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 2B antibody
    • Pyruvate dehydrogenase phosphatase 2 antibody
    • Pyruvate dehydrogenase phosphatase catalytic subunit 2 antibody
    • pyruvate dehydrogenase phosphatase isoenzyme 2 antibody
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 2 antibody
    see all

Images

  • All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Recombinant human PDP2 protein (ab110358) at 0.01 µg
    Lane 3 : Recombinant Human PDP1/PDP protein (ab110357) at 0.01 µg
    Lane 4 : Recombinant human PDK1 protein (ab110359) at 0.01 µg
    Lane 5 : HHH (Human heart homogenate) at 20 µg
    Lane 6 : HLH (Human liver homogenate) at 20 µg
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
    Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 5 minutes


    Predicted mature band size: 52 kDa
    Observed band size: 52 kDa

    Note: The antibody recognizes both the mature (52 kDa) and the immature (60 kDa) versions of PDP2 protein. The immunogen was mature PDP2 protein, lacking the first 66 amino acids which are used as a transit peptide for mitochondrial targeting. The antibody produces a band against the endogenous protein at the mature weight in tissue homogenates, but not in cancer cells where the protein may fail to mature.

References

This product has been referenced in:

  • Ryan ZC  et al. 1a,25-dihydroxyvitamin D3mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells. Biochem Biophys Res Commun 496:746-752 (2018). WB . Read more (PubMed: 29366785) »
  • Ryan ZC  et al. 1a,25-Dihydroxyvitamin D3 Regulates Mitochondrial Oxygen Consumption and Dynamics in Human Skeletal Muscle Cells. J Biol Chem 291:1514-28 (2016). Read more (PubMed: 26601949) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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