Overview

  • Product name

    Anti-PDPK1 antibody [3H3D9]
    See all PDPK1 primary antibodies
  • Description

    Mouse monoclonal [3H3D9] to PDPK1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PDPK1 aa 457-556. (Expressed in E.coli).
    Database link: O15530

  • Positive control

    • WB: MCF7, HeLa, and U937 cell lysates; Human PDPK1 (aa 457-556) recombinant protein; PDPK1 (aa 457-556)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab234064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificity

    Appears to be expressed ubiquitously.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localization

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Images

  • All lanes : Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : U937 (human histiocytic lymphoma cell line) cell lysate

    Predicted band size: 63 kDa

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PDPK1 with ab234064 at 1/200 dilution (green) compared with a negative control (red).

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PDPK1 (green) using ab234064 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution + Human PDPK1 (aa 457-556) recombinant protein

    Predicted band size: 63 kDa



    Expected MW is 37.9 kDa.

  • All lanes : Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : PDPK1 (aa 457-556)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 63 kDa

References

ab234064 has not yet been referenced specifically in any publications.

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