Recombinant Anti-PDPK1 antibody [5/PDK1] (ab280085)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [5/PDK1] to PDPK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PDPK1 antibody [5/PDK1]
See all PDPK1 primary antibodies -
Description
Mouse monoclonal [5/PDK1] to PDPK1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K-562, 22Rv1, F9, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5/PDK1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 63 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 63 kDa. |
Target
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Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. -
Tissue specificity
Appears to be expressed ubiquitously. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Entrez Gene: 81745 Rat
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- SwissProt: O55173 Rat
- Unigene: 459691 Human
see all -
Alternative names
- 3 phosphoinositide dependent protein kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
see all
Images
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All lanes : Anti-PDPK1 antibody [5/PDK1] (ab280085) at 1/1000 dilution
Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 3 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 52-65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:30171166).
Exposure time: 3 minutes
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All lanes : Anti-PDPK1 antibody [5/PDK1] (ab280085) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 52-65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab280085 has not yet been referenced specifically in any publications.