Overview

  • Product name
  • Description
    Rabbit polyclonal to PDPK1
  • Host species
    Rabbit
  • Specificity
    This antibody is primarily directed against 60,000 MW human PDPK1 and is useful in determining its presence in various assays. It predominantly recognizes a 60 kDa band on Western blots.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Peptide sequence:

    RKIQEVWRQRYQS

    (amino acids 301-312).

Properties

Applications

Our Abpromise guarantee covers the use of ab2495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Western Blot: use at 1/750 to detect a 60 kD band.
    (previously used at this dilution to detect PDK 1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK 1.
    Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation.
  • Target

    • Function
      Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    • Tissue specificity
      Appears to be expressed ubiquitously.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    • Cellular localization
      Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK1 antibody
      • Pdpk1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PDPK2P antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Images

    • Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDK 1 detects 60 kDa human PDK-1 in lysates from myc-tagged human PDK-1 virus infected Sf9 cells.  A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection.  Other detection systems will yield similar results.

      Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDPK1 detects 60 kDa human PDPK1 in lysates from myc-tagged human PDPK1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.

    References

    ab2495 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer

    Thank you for your phone call. I have enquired with the originators of these two antibodies and for ab2808, rat skeletal muscle homogenate should be used as a positive control and for ab2495, the originator used myc-tagged human PDK-1 virus infected Sf9 cells to test the antibody. This is all the information that we have at this time, if you have any more questions please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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