Key features and details
- Rabbit polyclonal to PDPK1 (phospho S241)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PDPK1 (phospho S241) antibody
See all PDPK1 primary antibodies
DescriptionRabbit polyclonal to PDPK1 (phospho S241)
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Extracts from 293 and PC12 cell treated with EGF; HeLa cells, Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131098 was purified by affinity chromatography using epitope specific phosphopetide. Non phospho specific antibodies were removed by chromatography using non phosphopetide.
Our Abpromise guarantee covers the use of ab131098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 63 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Tissue specificityAppears to be expressed ubiquitously.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
- Information by UniProt
- 3 phosphoinositide dependent protein kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
All lanes : Anti-PDPK1 (phospho S241) antibody (ab131098) at 1/500 dilution
Lane 1 : 293 cell extract, untreated
Lane 2 : 293 cell extract treated with EGF
Lane 3 : PC12 cell extract, untreated
Lane 4 : PC12 cell extract treated with EGF
Predicted band size: 63 kDa
Immunohistochemical analysis of PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue, using ab131098 at a dilution of 1/50. Image on the right was preincubated with blocking peptide.
Immunofluorescence analysis of PDPK1 (phospho S241) in methanol fixed HeLa cells, using ab131098 at a dilution of 1/100.
ab131098 has not yet been referenced specifically in any publications.