Application
Western blot
Sample
Human Cell lysate - whole cell (293t)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
10 µg
Specification
293t
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Lane 2: 293 transfected PDK1 WT + Lambda phosphatase
Lane 3: 293T transfected PDK1 S393A
Exposure
5 second(s)
Bands
Specific: 60 kDa
Positive control
Lane 1: 293T transfected PDK1 WT
Abcam user community
Verified customer
Submitted Sep 07 2017