Recombinant Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PDPK1 (phospho S410) antibody [EPR6157]
See all PDPK1 primary antibodies -
Description
Rabbit monoclonal [EPR6157] to PDPK1 (phospho S410) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Serine 410 of Human PDPK1 (UniProt O15530).
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Positive control
- 293T cell lysate treated with Okadaic Acid and Calyculin A
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6157 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 63 kDa. |
Target
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Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. -
Tissue specificity
Appears to be expressed ubiquitously. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Entrez Gene: 81745 Rat
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- SwissProt: O55173 Rat
- Unigene: 459691 Human
see all -
Alternative names
- 3 phosphoinositide dependent protein kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
see all
Images
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![Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)](https://www.abcam.com/ps/products/133/ab133461/Images/ab133461-147093-anti-pdpk1-phospho-s410-antibody-epr6157-western-blot.jpg)
Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)All lanes : Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 293T cell lysate treated with Okadaic Acid and Calyculin A.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 63 kDa
Protocols
Datasheets and documents
References
ab133461 has not yet been referenced specifically in any publications.
