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RecombinantRabMAb

Recombinant Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)

  • Datasheet
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Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
  • Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6157] to PDPK1 (phospho S410)
  • Suitable for: WB
  • Reacts with: Human

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Recombinant human PDPK1 protein (ab60834)

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Overview

  • Product name

    Anti-PDPK1 (phospho S410) antibody [EPR6157]
    See all PDPK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6157] to PDPK1 (phospho S410)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 410 of Human PDPK1 (UniProt O15530).

  • Positive control

    • 293T cell lysate treated with Okadaic Acid and Calyculin A
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6157
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Alternative Versions

    • Anti-PDPK1 (phospho S410) antibody [EPR6157] - BSA and Azide free (ab248507)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human PDPK1 protein (ab60834)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    • Tissue specificity

      Appears to be expressed ubiquitously.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    • Cellular localization

      Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Target information above from: UniProt accession O15530 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5170 Human
      • Entrez Gene: 18607 Mouse
      • Entrez Gene: 81745 Rat
      • Omim: 605213 Human
      • SwissProt: O15530 Human
      • SwissProt: Q9Z2A0 Mouse
      • SwissProt: O55173 Rat
      • Unigene: 459691 Human
      • Unigene: 10504 Mouse
      • Unigene: 10905 Rat
      see all
    • Alternative names

      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK1 antibody
      • Pdpk1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PDPK2P antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Images

    • Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
      Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
      All lanes : Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate treated with Okadaic Acid and Calyculin A.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa

    • Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
      Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab133461? Please let us know so that we can cite the reference in this datasheet.

    ab133461 has not yet been referenced specifically in any publications.

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