RecombinantRabMAb

Recombinant Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)

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Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
  • Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6157] to PDPK1 (phospho S410)
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PDPK1 (phospho S410) antibody [EPR6157]
    See all PDPK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6157] to PDPK1 (phospho S410)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 410 of Human PDPK1 (UniProt O15530).

  • Positive control

    • 293T cell lysate treated with Okadaic Acid and Calyculin A

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

    • Tissue specificity

      Appears to be expressed ubiquitously.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

    • Cellular localization

      Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK1 antibody
      • Pdpk1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PDPK2P antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Images

    • Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
      Western blot - Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
      All lanes : Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate treated with Okadaic Acid and Calyculin A.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa

    • Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)
      Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461)

    References (0)

    Publishing research using ab133461? Please let us know so that we can cite the reference in this datasheet.

    ab133461 has not yet been referenced specifically in any publications.

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