Overview

  • Product name
    Anti-PDS5B antibody [2030D32a]
    See all PDS5B primary antibodies
  • Description
    Mouse monoclonal [2030D32a] to PDS5B
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant C terminal fragment from human PDS5B.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab60060 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2030D32a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 41 kDa (fragment), (predicted molecular weight: 165 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • AI646570 antibody
      • Androgen induced proliferation inhibitor antibody
      • Androgen-induced prostate proliferative shutoff associated protein AS3 antibody
      • APRIN antibody
      • AS3 antibody
      • AW212954 antibody
      • CG008 antibody
      • FLJ23236 antibody
      • KIAA0979 antibody
      • mKIAA0979 antibody
      • PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) antibody
      • Pds5b antibody
      • RP1 267P19.1 antibody
      • Sister chromatid cohesion protein PDS5 homolog B antibody
      see all

    Images

    • Anti-PDS5B antibody [2030D32a] (ab60060) + immunising recombinant protein

      Predicted band size: 165 kDa
      Observed band size: 41 kDa (why is the actual band size different from the predicted?)



      The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

    References

    ab60060 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab60060.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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