Overview

  • Product name

  • Description

    Rabbit polyclonal to PDX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide within Human PDX1 (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P52945

  • Positive control

    • WB: Jurkat, Raji, NCI-H929 and AsPC-1 whole cell extracts; Mouse liver and pancreas liver tissue extracts; Rat liver tissue extract. IHC-P: Rat pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.

Target

  • Function

    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity

    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease

    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications

    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Images

  • Paraffin-embedded rat pancreas tissue stained for PDX1 using ab227586 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-PDX1 antibody (ab227586) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 31 kDa



    12% SDS-PAGE gel.

  • Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Mouse pancreas tissue extract at 50 µg

    Predicted band size: 31 kDa



    12% SDS-PAGE gel.

  • Anti-PDX1 antibody (ab227586) at 1/1000 dilution + AsPC-1 whole cell extract at 30 µg

    Predicted band size: 31 kDa



    12% SDS-PAGE gel.

  • Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Rat liver tissue extract at 50 µg

    Predicted band size: 31 kDa



    12% SDS-PAGE gel.

  • Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Mouse liver tissue extract at 50 µg

    Predicted band size: 31 kDa



     12% SDS-PAGE gel.

References

ab227586 has not yet been referenced specifically in any publications.

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