Overview

  • Product name
  • Description
    Rabbit polyclonal to PDX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Recombinant fusion protein containing N-Terminal region of mouse Pdx1

Properties

Applications

Our Abpromise guarantee covers the use of ab47267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/5000.
WB 1/3000 - 1/5000. Predicted molecular weight: 31 kDa.
ICC/IF 1/2000.

See ICC-specific protocol in protocol tab.

IHC-P Use at an assay dependent concentration.

See IHC-specific protocol in protocol tab.

Target

  • Function
    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease
    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain
    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications
    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Images

  • Immunohistochemical analysis of murine pancreatic islets, staining PDX1 (red) with ab47267.

    Tissue was fixed in formalin, permeablized with 0.25% Triton X-100, and blocked in blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody overnight at 4°C and a fluorescent-conjugated anti-rabbit IgG was used as the secondary antibody.
  • ab47267 staining PDX1 in rat pancreatic tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. 

    See Abreview

  • ab47267 staining PDX1 in embryonic (day 13.5) mouse pancreas tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 10% serum for 30 minutes at 220C was performed. The sample was incubated with primary antibody (1/5000) in 10% serum for 16 hours in 220C. A biotin-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/250 as secondary antibody. A biotin/avidin/streptavidin linked to HRP system has been used.

    See Abreview

  • Immunostaining of islet-epithelial clusters from human foetal pancreata with ab47267 (green). Nuclei are visualised with DAPI (blue).
  • ab47267 staining PDX1 in human appendix tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxide block and 10 minutes for protein block). The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary. 

References

This product has been referenced in:
  • Yuan F  et al. Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic ß Cell Identity in db/db Mice. Evid Based Complement Alternat Med 2019:1232913 (2019). Read more (PubMed: 31015847) »
  • Barati G  et al. Differentiation of microfluidic-encapsulated trabecular meshwork mesenchymal stem cells into insulin producing cells and their impact on diabetic rats. J Cell Physiol 234:6801-6809 (2019). Read more (PubMed: 30317587) »
See all 52 Publications for this product

Customer reviews and Q&As

1-10 of 22 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

Currently we only sell this product in 100ul sizes, but if you are interested in placing a large bulk order (greater to 10 units) then we could look into a custom order.

If this is something that you would be interested in, please let me know and I will pass your information to our sales department.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na Citrate pH 6.0
Permeabilization
No
Specification
Pancreas
Fixative
10% NBF

Dr. David Burk

Verified customer

Submitted Nov 08 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (AML-193)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
Loading amount
50 µg
Specification
AML-193
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 03 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
No
Specification
pancreas
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 30 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH=9.0
Permeabilization
Yes - PBS with 3% Triton-100
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 22 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® iPSC Diff Day 5)
Permeabilization
No
Specification
iCell® iPSC Diff Day 5
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Sep 17 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (H9 cell line upon differentiation)
Specification
H9 cell line upon differentiation
Permeabilization
Yes - 0.01% Triton X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 22 2014

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I appreciate your patience, it has taken some time to obtain the testing protocol for you.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC-P and rat samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. Once I have this information, I hope I may be able to make some suggestions to improve the results.

I would appreciate if you could also provide an image which would help us to assess the results.

I have also copied below the testing IHC-P protocol from the originator which I hope will be useful. Please note that this would be a guideline only, and further individual optimization may often be required.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a No Primary control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results


IHC-P protocol:
Immunohistochemistry:

Day I



Dewax and rehydrate as normal.
Antigen Retrieval: Heat slides in 90 ml 10mM sodium citrate for 9 min. at 30% power in a 600 watt microwave. (Solution should reach 90-95O C but not boil.) Cool to room temp,. ˜30 min.
Wash in PBS, 3 x 2 min.
Quench endogenous peroxidase activity in 3%H2O2 for 20 min.
Wash in PBS, 3 x 2 min.
Drop on 5% normal goat serum to cover sample. Inc. 1h, RT. Use 50 ml/ml serum in PBS + 1% BSA.
Dilute a-pdx1 antibody 1:60,000 in PBS + 1%BSA. Shake off blocking solution and drop on antibody to cover sample. Inc. overnight, 4O C in humidity chamber.



Day II



Shake off the antibody solution and wash slides in PBS, 3 x 2 min.
Drop on biotinylated goat a-rabbit antibody (Diluted 1:1000 in PBS + 1%BSA) to cover samples. Incubate 1 h, RT.
After 2O Ab has been on samples for 30 min., make up ABC solution: Add 20 ml/ml reagent A + 20 ml/ml reagent B in PBS, and mix well (it has to sit for 30 min.).
After 1 h incubation, wash slides in PBS, 3 x 2 min.
Drop on ABC mixture to cover each sample, and incubate for 30 min. RT.
Wash slides in PBS, 3 x 2 min.
Drop on DAB mixture to cover samples, 1-5 min. at room temp. (DAB = 1 drop each of A, B, and C/ml dH2O; from Zymed Labs). Watch reaction on dissecting microscope until desired brown is attained.
Wash in dH2O, 3 x 1 min.
Counterstain with hematoxylin (or methyl green) 30 sec. – 1 min.
Wash in dH2O for 30 seconds (or in isopropanol if methyl green used).
Dehydrate as usual.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human embryonic stem cell derived-pancreatic endoc)
Specification
human embryonic stem cell derived-pancreatic endoc
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Youngjin Kim

Verified customer

Submitted Oct 25 2012

Answer

Thank you for contacting us.

The response to the inquiry you found contained proprietary information and was therefore not published. However, we did find that the immunogen used to create this product had a sequence similarity is 64% to chicken, and thus cross-reactivity is rather unlikely. We therefore cannot enroll this product in our 100% Abreview testing program for chicken sample.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 22 Abreviews or Q&A

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