Anti-PDX1 antibody (ab47267)
- Datasheet
- References (52)
- Protocols
Overview
-
Product nameAnti-PDX1 antibody
See all PDX1 primary antibodies -
DescriptionRabbit polyclonal to PDX1
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
-
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human
-
Immunogen
Recombinant fusion protein containing N-Terminal region of mouse Pdx1
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
PurityWhole antiserum
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/5000. | |
| WB | 1/3000 - 1/5000. Predicted molecular weight: 31 kDa. | |
| ICC/IF | 1/2000. See ICC-specific protocol in protocol tab. |
|
| IHC-P | Use at an assay dependent concentration. See IHC-specific protocol in protocol tab. |
Target
-
FunctionActivates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
-
Tissue specificityDuodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
-
Involvement in diseaseDefects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similaritiesBelongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Contains 1 homeobox DNA-binding domain. -
DomainThe Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y). -
Post-translational
modificationsPhosphorylated by the SAPK2 pathway at high intracellular glucose concentration. -
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 538927 Cow
- Entrez Gene: 493994 Dog
- Entrez Gene: 3651 Human
- Entrez Gene: 18609 Mouse
- Entrez Gene: 29535 Rat
- Omim: 600733 Human
- SwissProt: P52945 Human
- SwissProt: P52946 Mouse
see all -
Alternative names
- Glucose sensitive factor antibody
- Glucose-sensitive factor antibody
- GSF antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)Image from Zhang Y et al., PLoS One. 2012;7(5):e36675. Epub 2012 May 7. Fig 7.; doi:10.1371/journal.pone.0036675; May 7, 2012, PLoS ONE 7(5): e36675.Immunohistochemical analysis of murine pancreatic islets, staining PDX1 (red) with ab47267.
Tissue was fixed in formalin, permeablized with 0.25% Triton X-100, and blocked in blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody overnight at 4°C and a fluorescent-conjugated anti-rabbit IgG was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)This image is courtesy of Antibody Solutions Ltd.ab47267 staining PDX1 in rat pancreatic tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
-
Immunohistochemistry (Frozen sections) - Anti-PDX1 antibody (ab47267)This image is a courtesy of Anonymous Abreviewab47267 staining PDX1 in embryonic (day 13.5) mouse pancreas tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 10% serum for 30 minutes at 220C was performed. The sample was incubated with primary antibody (1/5000) in 10% serum for 16 hours in 220C. A biotin-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/250 as secondary antibody. A biotin/avidin/streptavidin linked to HRP system has been used.
-
Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (ab47267)Immunostaining of islet-epithelial clusters from human foetal pancreata with ab47267 (green). Nuclei are visualised with DAPI (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)This image is courtesy of Antibody Solutions Ltd.ab47267 staining PDX1 in human appendix tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxide block and 10 minutes for protein block). The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary.
References
This product has been referenced in:
- Yuan F et al. Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic ß Cell Identity in db/db Mice. Evid Based Complement Alternat Med 2019:1232913 (2019). Read more (PubMed: 31015847) »
- Barati G et al. Differentiation of microfluidic-encapsulated trabecular meshwork mesenchymal stem cells into insulin producing cells and their impact on diabetic rats. J Cell Physiol 234:6801-6809 (2019). Read more (PubMed: 30317587) »
