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    pdx1-antibody-ab47267.pdf

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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like
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Anti-PDX1 antibody (ab47267)

  • Datasheet
  • SDS
Reviews (14)Q&A (8)References (69)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)
  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (ab47267)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)

Key features and details

  • Rabbit polyclonal to PDX1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PDX1 antibody
    See all PDX1 primary antibodies
  • Description

    Rabbit polyclonal to PDX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fusion protein containing N-Terminal region of mouse Pdx1

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Cancer
    • Growth factors
    • Insulin and insulin-like
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development
    • Metabolism
    • Types of disease
    • Diabetes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PDX1 protein (ab114175)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
1/2000.

See ICC-specific protocol in protocol tab.

IHC-P (5)
Use at an assay dependent concentration.

See IHC-specific protocol in protocol tab.

Notes
ICC/IF
1/2000.

See ICC-specific protocol in protocol tab.

IHC-P
Use at an assay dependent concentration.

See IHC-specific protocol in protocol tab.

Target

  • Function

    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity

    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease

    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications

    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P52945 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3651 Human
    • Omim: 600733 Human
    • SwissProt: P52945 Human
    • Unigene: 32938 Human
    • Alternative names

      • Glucose sensitive factor antibody
      • Glucose-sensitive factor antibody
      • GSF antibody
      • IDX 1 antibody
      • IDX-1 antibody
      • IDX1 antibody
      • Insulin promoter factor 1 antibody
      • Insulin promoter factor 1 homeodomain transcription factor antibody
      • Insulin upstream factor 1 antibody
      • IPF 1 antibody
      • IPF-1 antibody
      • IPF1 antibody
      • Islet/duodenum homeobox 1 antibody
      • Islet/duodenum homeobox-1 antibody
      • IUF 1 antibody
      • IUF-1 antibody
      • IUF1 antibody
      • MODY4 antibody
      • Pancreas/duodenum homeobox 1 antibody
      • Pancreas/duodenum homeobox protein 1 antibody
      • pancreatic and duodenal homeobox P antibody
      • PDX 1 antibody
      • PDX-1 antibody
      • PDX1 antibody
      • PDX1_HUMAN antibody
      • Somatostatin transactivating factor 1 antibody
      • Somatostatin-transactivating factor 1 antibody
      • STF 1 antibody
      • STF-1 antibody
      • STF1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)Image from Zhang Y et al., PLoS One. 2012;7(5):e36675. Epub 2012 May 7. Fig 7.; doi:10.1371/journal.pone.0036675; May 7, 2012, PLoS ONE 7(5): e36675.
      Immunohistochemical analysis of murine pancreatic islets, staining PDX1 (red) with ab47267.

      Tissue was fixed in formalin, permeablized with 0.25% Triton X-100, and blocked in blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody overnight at 4°C and a fluorescent-conjugated anti-rabbit IgG was used as the secondary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)This image is courtesy of Antibody Solutions Ltd.

      ab47267 staining PDX1 in rat pancreatic tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. 

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (ab47267)
      Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (ab47267)
      Immunostaining of islet-epithelial clusters from human foetal pancreata with ab47267 (green). Nuclei are visualised with DAPI (blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (ab47267)This image is courtesy of Antibody Solutions Ltd.

      ab47267 staining PDX1 in human appendix tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxide block and 10 minutes for protein block). The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary. 

    Protocols

    • Recommended IHC protocol with ab47267
    • ab47267 ICC/IF protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (69)

    Publishing research using ab47267? Please let us know so that we can cite the reference in this datasheet.

    ab47267 has been referenced in 69 publications.

    • Legøy TA  et al. Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling. Sci Rep 10:414 (2020). PubMed: 31942009
    • Lin JY  et al. In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals. Cell Discov 6:20 (2020). PubMed: 32284878
    • Pittala S  et al. The VDAC1-based R-Tf-D-LP4 Peptide as a Potential Treatment for Diabetes Mellitus. Cells 9:N/A (2020). PubMed: 32093016
    • Li Z  et al. ZLY032, the first-in-class dual FFA1/PPARd agonist, improves glucolipid metabolism and alleviates hepatic fibrosis. Pharmacol Res 159:105035 (2020). PubMed: 32562818
    • Gooraninejad S  et al. Small Molecule Differentiate PDX1-Expressing Cells Derived from Human Endometrial Stem Cells on PAN Electrospun Nanofibrous Scaffold: Applications for the Treatment of Diabetes in Rat. Mol Neurobiol 57:3969-3978 (2020). PubMed: 32632606
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 14 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Na Citrate pH 6.0
    Permeabilization
    No
    Specification
    Pancreas
    Fixative
    10% NBF
    Read More

    Dr. David Burk

    Verified customer

    Submitted Nov 08 2018

    Western blot abreview for Anti-PDX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (AML-193)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
    Loading amount
    50 µg
    Specification
    AML-193
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 03 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-PDX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (pancreas)
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 30 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDX1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH=9.0
    Permeabilization
    Yes - PBS with 3% Triton-100
    Specification
    Pancreas
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 22 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PDX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iCell® iPSC Diff Day 5)
    Permeabilization
    No
    Specification
    iCell® iPSC Diff Day 5
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PDX1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Cell (H9 cell line upon differentiation)
    Specification
    H9 cell line upon differentiation
    Permeabilization
    Yes - 0.01% Triton X100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PDX1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human embryonic stem cell derived-pancreatic endoc)
    Specification
    human embryonic stem cell derived-pancreatic endoc
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1 % Triton x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Youngjin Kim

    Verified customer

    Submitted Oct 25 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-PDX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Specification
    Pancreas
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 06 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-PDX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Pancreas (embryonic day 13.5))
    Specification
    Pancreas (embryonic day 13.5)
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 08 2009

    Western blot abreview for Anti-PDX1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Ms Pancreas, Ms Skeletal Muscle, Ms Brain)
    Loading amount
    30 µg
    Specification
    Ms Pancreas, Ms Skeletal Muscle, Ms Brain
    Gel Running Conditions
    Reduced Denaturing (12.5% SDS PAGE)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted Nov 17 2008

    1-10 of 14 Abreviews

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