Product nameAnti-PDX1 antibody
See all PDX1 primary antibodies
DescriptionGuinea pig polyclonal to PDX1
Host speciesGuinea pig
Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fusion protein containing N-terminal sequence from mouse PDX1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab47308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20170518|
|IHC-P||Use at an assay dependent dilution.|
FunctionActivates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
Tissue specificityDuodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
Involvement in diseaseDefects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesBelongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Contains 1 homeobox DNA-binding domain.
DomainThe Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
modificationsPhosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
- Information by UniProt
- Glucose sensitive factor antibody
- Glucose-sensitive factor antibody
- GSF antibody
Immunohistochemical analysis of developing murine pancreas taken from wild-type (+/+) and SEL1L-null mice (-/-). PDX1 (green) was stained with ab47308.
Tissue was fixed with paraformaldehyde before freezing and sectioning. Sections were permeabilized with 0.2% Triton X-100 for 20 min and blocked with 5% normal donkey serum and 1% BSA in PBS at room temperature for at least 1 hour. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) overnight at 4°C. A Cy2®-conjugated donkey anti-guinea pig IgG (1/500) was used as the secondary antibody.
Immunohistochemical analysis of paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized mouse pancreas tissue labelling PDX1 with ab47308 at 1/200 dilution,followed by Goat Anti-guinea pig IgG conjugation Alexa Fluor®488 secondary antibody at 1/200 dilution.
Immunostaining of islets from 4 week-old mice with ab47308 guinea pig anti-PDX1 (red) and an anti-insulin antibody (green).
This product has been referenced in:
- Bru-Tari E et al. Pancreatic alpha-cell mass in the early-onset and advanced stage of a mouse model of experimental autoimmune diabetes. Sci Rep 9:9515 (2019). Read more (PubMed: 31266981) »
- Memon B et al. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Ther 9:15 (2018). IHC-P ; Human . Read more (PubMed: 29361979) »