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I tried the fixation you suggested 4% PFA at 4C for 2 hrs. Still the antibodies didn't work while the one from R&D worked.
Asked on Oct 19 2011
I am sorry to read that this is still not working for you. My guess is that the fixation is still too long at 2 hours, even with 4% vs. 10% PFA, or that there is lot-to-lot variability with regards to compatibility with formaldeyhe-based fixation. If you like, I will replace the antibody but I am concerned you will have the same result if I send ab47308. Are you interested in trying a different PDX1 antibody? We do not have another antibody raised in guinea pig but we have one from goat, ab47383, and another from rabbit, ab47267. If you do not want to try one of these (or any other primary antibody in our catalogue) free-of-charge, I can issue a credit or refund. I look forward to your reply.
Answered on Oct 19 2011