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Thank you for your quick response. We have goat anti-PDX, which works very well. So I do need antibody from different species. We have rabbit anti-PDX1 from Abcam in our lab, which I tried but didn't work ver well. However I don't know if it is because of the antibody itself or just because the antibody is too old. Do do you think I may get a fresh test sample of rabbit anti-PDX1? I can test it and decide which one to use.
Asked on Oct 20 2011
I assume you have the antibody ab47267, as that is the only rabbit anti-PDX1 in our catalogue that has been tested for ICC. We do not have samples of any of our antibodies but I can send a full vial as a replacement, perhaps from a lot different from what you have already tried. If the fresh vial gives a poor result again, we can send a different antibody if you like. Do you know the catalogue number of the rabbit PDX1 antibody that you have, and the lot number?
Answered on Oct 20 2011