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Thank you very much. I would like to get ab47267 rabbit anti-PDX1. The abcam rabbit anti-PDX1 we have is ab12045. Also, could you please let me know the fixation condition for ab47267?
Asked on Oct 21 2011
I have issued a free of charge replacement with the order number ****. This is for rabbit anti-PDX1, ab47267, to replace ab47308. The fixation is with 4% PFA for 5 minutes, followed by permeabilization with Triton X-100. The full protocol is below. Staining Cell cultures. 1. Rinse cells with PBS. 2. Fix in 4% paraformaldehyde in PBS for 5 minutes. 3. Wash 3 times with cold PBS for 5 minutes. 4. Permeablilize membrane with 0.2% Triton X for 10 minutes. 5. Wash twice with cold PBS for 5 minutes. 6. Block with 10% goat serum in PBS for 30 minutes. 7. Add primary antibody (1/5000) for 4 hours. 8. Wash 3 times for 10 minutes. 9. Add secondary goat anti-rabbit antibody (Alexa546) at 1/1000 for 1 hour. 10. Wash with PBS for 5 minutes. 11. wash with DAPI (1/10 000) for 10 minutes. 12. Wash with PBS for 10 minutes. 13. Mount with fluorescent mounting media. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.
Answered on Oct 21 2011