Recombinant
RabMAb

Recombinant Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

Overview

  • Product name

    Anti-PDX1 antibody [EPR22002] - BSA and Azide free
    See all PDX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22002] to PDX1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Mouse PDX1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P52946

  • Positive control

    • IHC-P: Mouse pancreas tissue.
  • General notes

    Ab234633 is the carrier-free version of ab219207. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234633 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

WB Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity

    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease

    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications

    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining on human pancreatic islet and pancreatic acini (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling PDX1 with ab219207 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining in Beta-TC-6 cells.

    Negative control: NIH/3T3 (PMID: 25271154).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

  • PFX1 was immunoprecipitated from 0.35 mg of Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate with ab219207 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219207 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Beta-TC-6 whole cell lysate 10 µg (Input). 
    Lane 2: ab219207 IP in Beta-TC-6 whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219207 in Beta-TC-6 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in rat pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in mouse pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab234633 has not yet been referenced specifically in any publications.

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