Overview

  • Product name
  • Description
    Rabbit polyclonal to PDXK.1
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for PDKX.1.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected within aa 1~100 of human PDXK.1.

  • Positive control
    • Mouse intestine tissue lysate and Hela cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab38208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
ELISA 1/1000.

Target

Images

References

This product has been referenced in:
  • Hao YJ  et al. Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents. Oncol Lett 10:1333-1338 (2015). WB . Read more (PubMed: 26622672) »
  • Missner E  et al. Off-target decoding of a multitarget kinase inhibitor by chemical proteomics. Chembiochem 10:1163-74 (2009). WB ; Human . Read more (PubMed: 19350611) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

That's my pleasure.

I have informed my colleagues from Finance onhow you would like usethe credit note.

If you have any further questions in the future please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

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Answer

Thank you for contacting us.

Your credit note ID is *****.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at mailto:creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice.

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Question

WB Questionnaire
1) Abcam product code ab 38208
2) Abcam order reference number or product batch number GR57485-1
3) Description of the problem The antibody doesn't work.
4) Sample preparation:
Type of sample (whole cell lysates, fraction, recombinant protein…): HeLa whole cell lysate
Lysis buffer
Protease inhibitors: PIC (Roche)
Phosphatase inhibitors: PMSF
Reducing agent
Boiling for ≥5 min? yes
Protein loaded ug/lane or cells/lane 109 cells/lane
Positive control: alpha-tubulin
Negative control: none

5) Percentage of gel: 10%
Type of membrane: hybond ECL
Protein transfer verified yes with poinceau
Blocking agent and concentration 5% milk in TBS-T or 5% milk in PBS-T
Blocking time 45'
Blocking temperature room temperature

6) Primary antibody (If more than one was used, describe in “additional notes”) :
Concentration or dilution 1:100
Diluent buffer TBS-T or PBS-T
Incubation time over night
Incubation temperature: 4°

7) Secondary antibody: anti-rabbit
Species: horse radish
Reacts against: rabbit
Concentration or dilution 1:5000
Diluent buffer
Incubation time 60'
Incubation temperature: room temperature
Fluorochrome or enzyme conjugate: hrp

8) Washing after primary and secondary antibodies:
Buffer TBS-T or PBS-T
Number of washes 10' for two times

9)Detection method : ECL
10) How many times have you run this staining? 1 time
Do you obtain the same results every time? yes
What steps have you altered to try and optimize the use of this antibody? I use PBS-T instead of TBS-T

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Answer

Thank you for taking time to complete our questionnaire and for contacting us.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.Since the only lot of ab38208 we currently have in stock is the same as the one you originally received, I can offer you as a free of charge replacement, one unit of an alternative anti-pdxk.1 such as https://www.abcam.com/ab17449, https://www.abcam.com/ab28970 or https://www.abcam.com/ab119051.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding theprotocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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