Overview

  • Product name

    Anti-PDXP antibody
  • Description

    Rabbit polyclonal to PDXP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (PWHPLSDGSR TPGTGSLAAA VETASGRQAL VVGKPSPYMF ECITENFSID) of human PDXP (NP_064711).

  • Positive control

    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho-tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP.
  • Tissue specificity

    Ubiquitous. Highly expressed in all the regions of central nerve system except the spinal cord. Also expressed at high level in liver and testis. In fetus, it is weakly expressed in all organs except brain.
  • Sequence similarities

    Belongs to the HAD-like hydrolase superfamily.
  • Cellular localization

    Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Cell projection, lamellipodium membrane. Cell membrane. Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Diffusely distributed throughout the cytosol during pro-metaphase and metaphase. Detected at the dynamic cell poles during telophase. Detected at the cleavage furrow and contractile ring during cytokinesis. Transiently detected at the plasma membrane in late stages of cytokinesis. Detected at the midbody.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chronophin antibody
    • CIN antibody
    • Pdxp antibody
    • PLP antibody
    • PLP phosphatase antibody
    • PLPP antibody
    • PLPP_HUMAN antibody
    • Pyridoxal (pyridoxine, vitamin B6) phosphatase antibody
    • Pyridoxal phosphate phosphatase antibody
    • Rbp1 antibody
    • Reg I binding protein 1 antibody
    • RP1 37E16.5 antibody
    see all

Images

  • Anti-PDXP antibody (ab86422) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted at 1/50000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa

References

ab86422 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86422.
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