Anti-PDZ-RhoGEF antibody (ab110059)
Key features and details
- Rabbit polyclonal to PDZ-RhoGEF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDZ-RhoGEF antibody -
Description
Rabbit polyclonal to PDZ-RhoGEF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human PDZ-RhoGEF (N terminal).
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Positive control
- A549 and COLO cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab110059 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 168 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 168 kDa. |
Target
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Function
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 1 PH domain.
Contains 1 RGSL (RGS-like) domain. -
Domain
The poly-Pro region is essential for plasma membrane localization upon stimulation. -
Cellular localization
Cytoplasm. Membrane. Translocated to the membrane upon stimulation. - Information by UniProt
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Database links
- Entrez Gene: 9826 Human
- Entrez Gene: 213498 Mouse
- Entrez Gene: 78966 Rat
- Omim: 605708 Human
- SwissProt: O15085 Human
- SwissProt: Q9ES67 Rat
- Unigene: 516954 Human
- Unigene: 160580 Rat
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Alternative names
- ARHGB_HUMAN antibody
- ARHGEF 11 antibody
- ARHGEF11 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab110059 has been referenced in 3 publications.
- Chandan NR et al. A network of Gαi signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEF. Sci Signal 15:eabi9869 (2022). PubMed: 35041463
- Zhang W et al. Transgenerational Obesity and Alteration of ARHGEF11 in the Rat Liver Induced by Intrauterine Hyperglycemia. J Diabetes Res 2019:6320839 (2019). PubMed: 31612150
- Zhang W et al. ARHGEF11 affecting the placental insulin signaling pathway in fetal macrosomia of normal glucose tolerance pregnant women. Placenta 63:7-14 (2018). WB, IHC-P . PubMed: 29486856