Product nameAnti-PDZK1 antibody
See all PDZK1 primary antibodies
DescriptionRabbit polyclonal to PDZK1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Chinese hamster
- Human, mouse and rat liver lysate; Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500. Predicted molecular weight: 57 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
FunctionA scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
Tissue specificityExpression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas.
Sequence similaritiesBelongs to the NHER family.
Contains 4 PDZ (DHR) domains.
DomainThe PDZ 2 and 3 domains seem to be involved in the interaction with SLC26A3.
Interaction with the C-terminus of CFTR could be mediated through independent binding of PDZ 1, 3 and 4 domains.
The PDZ 1 and 3 domains seem to be involved in the interaction with SLCO1A1.
The PDZ 1 domain interacts with BCR.
Cellular localizationCytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
- Information by UniProt
- 1700023D20Rik antibody
- 2610507N21Rik antibody
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Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121248 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-PDZK1 antibody (ab121248)
Lane 1 : Caco-2 cells transfected with control siRNA
Lane 2 : Caco-2 cells transfected with target specific siRNA probe #1
Predicted band size: 57 kDa
Loading control: Anti-GAPDH.
All lanes : Anti-PDZK1 antibody (ab121248) at 1/250 dilution
Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : RT-4 lysate
Lane 5 : Liver lysate
Lane 6 : Tonsil lysate
Predicted band size: 57 kDa
Immunohistochemical staining of paraffin embedded Human kidney, using 1/150 ab121248, shows strong cytoplasmic positivity in cells in tubules.
ab121248 has not yet been referenced specifically in any publications.