Overview

  • Product name

    Anti-PDZK1 antibody [EPR3751]
    See all PDZK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3751] to PDZK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PDZK1 aa 450-550. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal liver, fetal kidney and kidney tissue lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3751
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
    • Tissue specificity

      Expression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas.
    • Sequence similarities

      Belongs to the NHER family.
      Contains 4 PDZ (DHR) domains.
    • Domain

      The PDZ 2 and 3 domains seem to be involved in the interaction with SLC26A3.
      Interaction with the C-terminus of CFTR could be mediated through independent binding of PDZ 1, 3 and 4 domains.
      The PDZ 1 and 3 domains seem to be involved in the interaction with SLCO1A1.
      The PDZ 1 domain interacts with BCR.
    • Cellular localization

      Cytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1700023D20Rik antibody
      • 2610507N21Rik antibody
      • 4921513F16Rik antibody
      • AI267131 antibody
      • AI314638 antibody
      • AL022680 antibody
      • C terminal linking and modulating protein antibody
      • CAP70 antibody
      • CFTR associated protein of 70 kDa antibody
      • CFTR associated protein, 70-KD antibody
      • CFTR-associated protein of 70 kDa antibody
      • CLAMP antibody
      • D3Ertd537e antibody
      • Dietary Pi-regulated RNA-1 antibody
      • Diphor-1 antibody
      • mPDZK1 antibody
      • Na(+)/H(+) exchange regulatory cofactor NHE-RF3 antibody
      • Na(+)/H(+) exchanger regulatory factor 3 antibody
      • Na/Pi cotransporter C-terminal-associated protein 1 antibody
      • Na/Pi cotransporter C-terminal-associated protein antibody
      • NaPi Cap1 antibody
      • NaPi-Cap1 antibody
      • NaPiCap1 antibody
      • NHERF 3 antibody
      • NHERF-3 antibody
      • NHERF3 antibody
      • NHRF3_HUMAN antibody
      • OTTHUMP00000015572 antibody
      • PDZ domain containing 1 antibody
      • PDZ domain containing protein 1 antibody
      • PDZ domain-containing protein 1 antibody
      • PDZD1 antibody
      • PDZK1 antibody
      • Sodium hydrogen exchanger regulatory factor 3 antibody
      • Sodium-hydrogen exchanger regulatory factor 3 antibody
      see all

    Images

    • All lanes : Anti-PDZK1 antibody [EPR3751] (ab92491) at 1/5000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-PDZK1 antibody [EPR3751] (ab92491) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : T47-D (human mammary gland ductal carcinoma) cell lysate
      Lane 4 : Caco-2 (human colorectal adenocarcinoma) cell lysate
      Lane 5 : HeLa (human cervix adenocarcinoma) cell lysate
      Lane 6 : MCF-7 (human breast carcinoma) cell lysate

      Lysates/proteins at 20 µg/ml per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa


      Exposure time: 5 seconds

    References

    This product has been referenced in:

    • Ferreira C  et al. The scaffold protein PDZK1 modulates expression and function of the organic anion transporting polypeptide 2B1. Eur J Pharm Sci 120:181-190 (2018). IHC . Read more (PubMed: 29752999) »
    See 1 Publication for this product

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