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  1. Link

    pe--r-phycoerythrin-conjugation-kit-lightning-link-ab102918.pdf

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Reviews (1)Q&A (7)References (93)

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Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit Lightning-Link
  • Conjugation - R-Phycoerythrin Conjugation (ab102918)
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling ADRB2 antibody for Flow Cytometry
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling F(ab')2 antibody for Bead array
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling  anti-MUC1 SP antibodies FACS
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling SPmAb-6 antibody for Imagestream analysis
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling a panel of antibodies for Microsphere immunoassay based on Luminex
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling A20 antibody for FACS
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-mouse insulin antibody for Flow Cytometry
  • PE conjugation kit used to label DDR2 antibody for Flow Cytometry
  • PE conjugation to an anti-chymase antibody with ab102918 PE Conjugation Kit
  • Conjugation of recombinant influenza A H1N1 NP protein with PE or APC fluorochromes using ab102918
  • Flow cytometry using antibody conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102918)

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Overview

  • Product name

    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®
  • Product overview

    R-PE Conjugation Kit/ R-PE Labeling Kit ab102918 uses a simple and quick process for PE labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


    To conjugate an antibody to R-PE using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The PE conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to R-PE.


    Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

  • Notes

    This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® R-PE Labeling Kit. 703-0004 is the same as the 3 mg size. 703-0003 is the same as the 5 x 600 ug size. 703-0010 is the same as the 3 x 60 ug size. 703-0030 is the same as the 3 x 10 ug size. 703-0015 is the same as the 600 µg size. 703-0005 is the same as the 60 µg size.

    Amount and volume of antibody for conjugation to R-PE

    Kit size  Recommended 
    amount of antibody
     

    Maximum antibody 
    volume1 

    3 x 10 µg 3 x 10 µg 3 x 10 µL
    60 µg 1 x 60 µg 1 x 60 µL
    3 x 60 µg 3 x 60 µg 3 x 60 µL
    600 µg 1 x 600 µg 1 x 600 µL
    5 x 600 µg 5 x 600 µg 5 x 600 µL
    3 mg 1 x 3 mg 1 x 3 mL

    The selling size of this product is now based on the amount of antibody that can be conjugated with the kit; the amount of antibody advised that can be used with the kit has also been updated to reflect what will give the best conjugation results. The quantity and formulation of reagents provided have not changed, if you have been previously using the kit successfully with a different amount of antibody, there is no need to change the way that you are using the kit.

    1Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1% BSA 50% glycerol
    0.1% sodium azide PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%

    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

    Storing and handling conjugation kits

    Lyophilized Lightning-Link® components are hygroscopic.

    Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 60 µg 600 µg 5 x 600 µg 3 x 60 µg 3 x 10 µg 1 x 3 mg
    ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 1200µl 1 x 200µl 1 x 200µl 1 x 1200µl
    ab274296 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 1200µl 1 x 200µl 1 x 200µl 1 x 1200µl
    ab274122 - R-PE mix 1 x 60µg 1 x 600µg 5 x 600µg 3 x 60µg 3 x 10µg 1 x 3mg
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Kits/ Lysates/ Other
    • Kits
    • Conjugation Kits
    • Phycoerythrin
  • Alternative names

    • R Phycoerythrin
    • RPE

Associated products

  • Related Products

    • Antibody Concentration And Clean-Up Kit (ab102778)
    • Antibody Purification Kit (Protein A) (ab102784)

Images

  • Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit Lightning-Link
    Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit Lightning-LinkImage from Robinson, Andrew P., et al., PloS one, 9(9):e107649. doi: 10.1371/journal.pone.0107649. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Robinson, Andrew P., et al used PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) as part of characterizing oligodendroglial populations. They used the kit to conjugate PE / R-Phycoerythrin to Mouse monoclonal anti-GALC antibody for use in flow cytometry.
    SJL/J mice were immunized with PLP139-151 and scored daily for clinical disease. A cohort of SJL/J mice was sacrificed, and spinal cords were analyzed by flow cytometry (n=5). (A) Cells were distinguished from debris by forward and side scatter then singlet cells were gated. Live cells were gated by dead cell exclusion, and CNS resident cells were identified as CD45- or CD45low. (B) Oligodendroglial cells were defined by double positive staining: A2B5+PDGFRalpha+ early OPCs, A2B5+NG2+ intermediate OPCs, NG2+O4+ late OPCs, O4+MOG+ pre-myelinating oligodendrocytes, and GALC+MOG+ mature oligodendrocytes.

  • Conjugation - R-Phycoerythrin Conjugation (ab102918)
    Conjugation - R-Phycoerythrin Conjugation (ab102918)
  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling ADRB2 antibody for Flow Cytometry
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling ADRB2 antibody for Flow CytometryImage from Bigler MB et al., PloS One, 10(12):e0145635. Fig 3.; doi: 10.1371/journal.pone.0145635. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Bigler MB et al. used ab102918 to assess expression of the epinephrine receptor (ADRB2) on CD3- CD56+ lymphocytes by flow cytometry.

  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling F(ab')2 antibody for Bead array
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling F(ab')2 antibody for Bead arrayImage from Ayoglu B et al., PLoS One 9(5):e96403. Fig 2.; doi: 10.1371/journal.pone.0096403. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Ayoglu B et al. used ab102918 to detect complement activation driven C3 deposition on beads.

    For measuring the background, classical, lectin & alternative and only alternative pathway activation, a serially diluted serum sample (1∶1–1∶160) was applied to empty, human IgG. The assay buffer for serum dilution contained either Ca2+-Mg2+, which promotes complement activation, or EDTA, which blocks complement activation. Plot displays for each serum dilution the respective median fluorescence intensity (MFI) value against varying concentrations of human IgG.

  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling  anti-MUC1 SP antibodies FACS
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-MUC1 SP antibodies FACSImage from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 3.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Kovjazin R et al. used ab102918 to evaluate the cell surface presence of the MUC1 SP domain by FACS.

    The cell surface presence of the MUC1 SP domain was evaluated by gated FACS analysis on PC cells in fresh BM aspirates obtained from MM patients (A–E) and normal naive sample (F–H).

  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling SPmAb-6 antibody for Imagestream analysis
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling SPmAb-6 antibody for Imagestream analysisimage from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 2.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Kovjazin R et al. used ab102918 for detection of MUC1 SP on the membrane of MUC1-positive tumor cells.

  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling a panel of antibodies for Microsphere immunoassay based on Luminex
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling a panel of antibodies for Microsphere immunoassay based on LuminexImage from Charlermroj R et al., PloS One, 8(4):e62344. Fig 2.; doi: 10.1371/journal.pone.0062344. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Charlermroj R et al. used ab102918 as part of an experiment studying four different plant pathogens: a fruit blotch bacterium Acidovorax avenae subsp. citrulli (Aac), chilli vein-banding mottle virus (CVbMV, potyvirus), watermelon silver mottle virus (WSMoV, tospovirus serogroup IV) and melon yellow spot virus (MYSV, tospovirus).

    (A-G) X-axis is antibody-coated microsphere and y-axis is median fluorescent intensity (MFI) from each RPE-labeled antibody. (H) Summary of selected antibody pair sets for the detection of the four plant pathogens.

  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling A20 antibody for FACS
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling A20 antibody for FACSImage from Hjelmeland AB et al., PLoS Biol., 8(2):e1000319. Fig 2.; doi: 10.1371/journal.pbio.1000319.Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Hjelmeland AB et al. used ab102918 as part of an experiment studying the mechanisms that cause A20 regulator to have differential effects on tumor growth and cancer cell behaviors.

    They used the PE labelling kit to conjugate phycoerythrin to A20 antibody for use in FACS.

    FACS plots are shown for T08-837 (A) and CW468 (B).

  • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-mouse insulin antibody for Flow Cytometry
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-mouse insulin antibody for Flow CytometryImage from Kalis, M et al., PLoS One, 6(12):e29166. Fig 5.; doi: 10.1371/journal.pone.0029166. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Kalis M et al. used ab102918 as part of examining beta-cells deletion of Dicer1.

    They used the kit to conjugate PE to anti-mouse insulin antibody for use in flow cytometry.

    Islet cells suspension from 50 islets from 8 weeks old littermates and RIP-Cre Dicer1Δ/Δ mice were stained for insulin and glucagon and analyzed by flow cytometry. Side scatter (SSC) and forward scatter (FSC) analysis of islet cells and the respective gate used to identify live cells. Littermate control islet cells contained significantly more insulin-positive cells (P<0.001) and less glucagon-positive cells (P<0.05) than the RIP-Cre Dicer1Δ/Δ islet cells. Representative of 3 independent experiments.

  • PE conjugation kit used to label DDR2 antibody for Flow Cytometry
    PE conjugation kit used to label DDR2 antibody for Flow CytometryBagchi RA et al. BMC Biol. 2016 Mar 17;14:21 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    RA Bagchi et al used PE conjugation kit / PE labeling kit ab102918 as part of examining the conversion of fibroblasts to myofibroblasts. They used the PE labeling kit to conjugate phycoerythrin to a DDR2 antibody for use in flow cytometry.

    Charts are forward-scatter plots illustrating flow cytometry analysis of cardiac cells from WT and scleraxis KO mice. Left column, unstained cells; center column, stained cells from WT tissue; right column, stained cells from scleraxis KO tissue. Results are representative of assessments from n = 3 independent tissue samples. Purple outline denotes labeled cells, and is derived from unstained plots.

  • PE conjugation to an anti-chymase antibody with ab102918 PE Conjugation Kit
    PE conjugation to an anti-chymase antibody with ab102918 PE Conjugation KitImage from Rönnberg E et al., Frontiers in immunology., 10, 1361.. Fig 4C.; doi: 10.3389/fimmu.2019.01361 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/.

    Ronnberg E et al. conjugated PE to an anti-chymase antibody with ab102918 PE Conjugation Kit as part of investigating the role of IL-33 in mast cells.
    The mean fluorescence intensity of chymase expression in cells was measured by intra-cellular flow cytometry staining and normalized to the respective isotype control.

    This data shows that there was no significant change in the expression on chymase in cord blood-derived mast cells (CBMC) when treated with different combinations of IL-33 and TSLP.

     

  • Conjugation of recombinant influenza A H1N1 NP protein with PE or APC fluorochromes using ab102918
    Conjugation of recombinant influenza A H1N1 NP protein with PE or APC fluorochromes using ab102918Image from Tan HX et al., Frontiers in immunology., 10, 611.. Fig 4A.; doi: 10.3389/fimmu.2019.00611 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Tan H-X et al. labeled recombinant influenza A H1N1 NP protein with PE or APC fluorochromes using ab102918 PE / R-Phycoerythrin Conjugation Kit and ab201807 APC Conjugation Kit.

    Representative HA and NP probe staining in flow cytometry of GC B cells (B220+ IgD- CD38lo GL7+) isolated from lung inducible bronchus-associated tissues (iBALT), mediastinal LN (MLN), and spleen of mice at d35 post-infection with A/Puerto Rico/8/34 (PR8).

  • Flow cytometry using antibody conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102918)
    Flow cytometry using antibody conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102918)This image is courtesy of an anonymous Abreview.

    Flow cytometry histogram showing integrin beta-3 positive population of platelets from wildtype and knockout mice. Integrin beta-3 antibody was conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102918). Flow cytometry was performed using platelets from wild type and integrin beta-3 knockout mice. Mice that expressed beta-3 (shown in red) had a clear shift in FL-2 fluorescence over beta-3 knockout mice (shown in black).

Protocols

  • Protocol Booklet
  • Protocol Booklet - Chinese Version

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (93)

Publishing research using ab102918? Please let us know so that we can cite the reference in this datasheet.

ab102918 has been referenced in 93 publications.

  • Guito JC  et al. Asymptomatic Infection of Marburg Virus Reservoir Bats Is Explained by a Strategy of Immunoprotective Disease Tolerance. Curr Biol 31:257-270.e5 (2021). PubMed: 33157026
  • Bonser LR  et al. Flow-Cytometric Analysis and Purification of Airway Epithelial-Cell Subsets. Am J Respir Cell Mol Biol 64:308-317 (2021). PubMed: 33196316
  • van der Donk LEH  et al. Separate signaling events control TCR downregulation and T cell activation in primary human T cells. Immun Inflamm Dis 9:223-238 (2021). PubMed: 33350598
  • Tan TK  et al. A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses. Nat Commun 12:542 (2021). PubMed: 33483491
  • Zhang C  et al. NLRP3 inflammasome induces CD4+ T cell loss in chronically HIV-1-infected patients. J Clin Invest 131:N/A (2021). PubMed: 33720048
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Minimal handling, and it worked!!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The kit was extremely easy to use and effective despite the very small amount of antibody labeled (60 ug)- minimal handling, and it worked!!
Flow cytometry histogram showing integrin beta-3 positive population of platelets from wildtype and knockout mice. Integrin beta-3 antibody was conjugated using EasyLink R-Phycoerythrin conjugation kit (ab102919). Flow cytometry was performed using platelets from wild type and integrin beta-3 knockout mice. Mice that expressed beta-3 (shown in red) had a clear shift in FL-2 fluorescence over beta-3 knockout mice (shown in black).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 24 2019

Question

Hi! I was simply wondering if a small amount ( ie 0.1 M) of Tris-Glycine would be a compatible additive during conjugation? Thanks!

Read More

Abcam community

Verified customer

Asked on Jun 10 2014

Answer

Compounds containing amines (such as Tris) compete with the antibody or other protein for the R-Phycoerythrin. Unusually for an amine, Tris has little effect on conjugation efficiency as long as the concentration is 20mM or less. So, if 100 mM (0.1 M) is going to be the final concentration of the Tris, the conjugation may not work.

Read More

Tom Ruyle

Abcam Scientific Support

Answered on Jun 10 2014

Question

Objective: I am interested in one of your R-PE conjugation kit for FACS antibodies.

Previously, we ordered Integrin B1 antibody ((12G10)(ab30394)) for FACS analysis.

Because your antibody is a purified one, I plan to conjugate R-PE dye to the antibodies and I found your R-PE conjugation kit (ab 102918).

I want to know whether I can use your R-PE conjugation kit-produced labeled antibodies directly to the sample
or I should use extra antibody purification step (such as column purification) before FACS analysis.

Please let me know ASAP.

Read More

Abcam community

Verified customer

Asked on Jan 31 2013

Answer

Thank you for contacting us.

We have the same integrin beta 1 antibody [12G10] that is FITC conjugated as ab150002, just so you know, but we do not have an R-PE conjugated version.

But if you would like to conjugate the ab30394 to R-PE using our EasyLink kit ab102918, that would definitely work. Once the antibody is conjugated using the kit, it is ready to use and no further purification is necessary.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 31 2013

Question

I had a question regarding product #ab102918 (EasyLink R-Phycoerythrin Conjugation Kit). I intend to use this product in a process which necessitates the use of only Animal-Free Products. Is this product animal component free?

Read More

Abcam community

Verified customer

Asked on Jan 03 2013

Answer

Thank you for contacting us.


I can confirm that the R-Phycoerythrin (RPE) conjugation kit is indeed free of animal components.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 03 2013

Question

Hello,

I need to stain surface FREM2 protein on MCF7 cell line for following application in flow cytometry experiment. I found Abcam Anti-FREM2 antibody (ab75803) and EasyLink R-Phycoerythrin Conjugation Kit (3 x 100µg R-PE) (ab102918) – are these products suitable for flow cytometry? There’s 1% BSA in FREM2 antibody’s solution – will BSA interfere with R-PE labeling because of aminogroups?

Read More

Abcam community

Verified customer

Asked on Dec 10 2012

Answer

Thank you for contacting us. We have not tested ab75803 in Flow Cytometry and do not have any data regarding suitability in this application. You would have to purify the antibody before conjugating it to remove the BSA or the BSA may be conjugated.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Dec 11 2012

Question

I would like to use EasyLink for FITC or PE conjugation of my antibodies (I would like to use them for flow) but my antibodies concentration is lower than 1 mg/ml (between 0.1 and 0.5 mg/mL). Will it works? Do you have any previous experience?

Read More

Abcam community

Verified customer

Asked on Jun 25 2012

Answer

Thank you for contacting us.

The EasyLink conjugations require antibody concentrations of at least 1mg/ml. Below that, the conjugation becomes inefficient and you risk wasting the conjugate, not to mention the antibody. For antibodies of lower concentrations, we recommend bringing them up to 1mg/ml or more with a concentration columns. We have two sizes: one column or three columns.

Click here (or use the following: https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html).

Click here (or use the following: https://www.abcam.com/Antibody-Concentration-And-Clean-Up-Kit-ab102778.html).

These are very easy to use, and ensure effective conjugations. Please let me know if you have any questions.

Read More

Abcam Scientific Support

Answered on Jun 25 2012

Question

Inquiry: I have a question, I know the product can be used to conjugate antibodies, but can it be used to conjugate proteins? Thank you.

Read More

Abcam community

Verified customer

Asked on May 02 2012

Answer

Thank you for contacting us with your question.

Ab102918 has been developed to conjugate antibodies, however it targets available amine groups, so it can conjugate proteins and peptides provided there is at least one aminegroup (lysine) available.

For this reaction to be successful, the same restrictions do apply. The protein should be purified and in an amine free buffer (to prevent conjugation of buffer constituents), and the concentration should be at least 0.5 mg/mL. Depending on the protein size, the conjugation ratio can be very different than what is stated on the datasheet. For instance, many small peptides can conjugate to one molecule of PE, whereas we would expect an antibody to PE ratio of 1:1.

I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on May 02 2012

Question


Request you to please let me know whether abcam
can supply the following antibodies (reactivity:
human)
1. Fluorescein isothiocyanate (FITC)-conjugated
antibody against the melanosome-specific protein
gp100
2. Phycoerythrin (PE)-conjugated antibody against
the keratinocyte-specific marker cytokeratin.
3. PE conjugated antibody against collagen 1, type
1
All are used for flowcytometry. Antibodies 1&2
will be used together

Read More

Abcam community

Verified customer

Asked on Feb 21 2012

Answer

Thank you for contacting us.

We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: https://www.abcam.com/PE-pan-Cytokeratin-antibody-C-11-ab52460.html).

For collagen I, we have only one unconjugated antibody that has been tested in flow cytometry. We do, however, have a kit that enables one to easily conjugate the antibody to PE.

Collagen I - ab23446
EasyLink PE - ab102919 or ab102918

For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin). This should allow you to apply both antibodies to the same sample without cross-reaction of the secondary anti-IgG2b with mouse IgG1.

Click here (or use the following: https://www.abcam.com/Melanoma-gp100-antibody-OTI7E3-ab117853.html).

Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG2b-heavy-chain-FITC-preadsorbed-ab98702.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answered on Feb 21 2012

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