PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)

Reviews (2)Q&A (5)References (6)
Flow Cytometry - PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)

    Key features and details

    • PE Mouse monoclonal [AD1.1.10] to 6X His tag®
    • Suitable for: Flow Cyt
    • Reacts with: Species independent
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-6X His tag® antibody [AD1.1.10]
      See all 6X His tag® primary antibodies

    • Description

      PE Mouse monoclonal [AD1.1.10] to 6X His tag®

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide HHHHHH tag - tagged

    • General notes

      Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.013% Sodium azide
      Constituents: 1.64% Sodium phosphate, 1.71% Sucrose, 0.1% BSA, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

    • Clonality

      Monoclonal

    • Clone number

      AD1.1.10

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab72467 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/50 - 1/100.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Relevance

      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    • Cellular localization

      Depends upon the localization of the parent protein tagged with hexahistidine.

    • Alternative names

      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    Images

    • Flow Cytometry - PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
      Flow Cytometry - PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)This image is courtesy of an Anonymous Abreview.
      BALB-3T3 cells, which express moderate amounts of EGFR, were incubated with a His6-tagged anti-EGFR fibronectin domain. The labeled cells were washed and incubated with 50 microliters of 1:15 diluted PE-conjugated anti-His6 antibody. Cells were washed and analyzed by flow cytometry. A control without fibronectin domain was also analyzed. Image: Dashed black line is control without fibronectin. Solid blue line is sample with His6-tagged anti-EGFR fibronectin domain.

      See Abreview

    References (6)

    Publishing research using ab72467? Please let us know so that we can cite the reference in this datasheet.

    ab72467 has been referenced in 6 publications.

    • Murata K  et al. Landscape mapping of shared antigenic epitopes and their cognate TCRs of tumor-infiltrating T lymphocytes in melanoma. Elife 9:N/A (2020). PubMed: 32314731
    • Ju B  et al. Human neutralizing antibodies elicited by SARS-CoV-2 infection. Nature 584:115-119 (2020). PubMed: 32454513
    • Liu W  et al. Structural Basis of CD160:HVEM Recognition. Structure 27:1286-1295.e4 (2019). PubMed: 31230945
    • Ruella M  et al. Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell. Nat Med 24:1499-1503 (2018). PubMed: 30275568
    • Webb AJ  et al. A protease-based biosensor for the detection of schistosome cercariae. Sci Rep 6:24725 (2016). PubMed: 27090566
    • Rothe A  et al. The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor activity against colon carcinoma. Int J Cancer 134:2829-40 (2014). PubMed: 24242212

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Question



    We have purified recombinant protein containing polihystidine tag in a solution extracted from E.coli cells. We want to confirm that our solution indeed contains those proteins so that we will use it in our experiments. One of our collaborators has your
    Anti-6X His tag® antibody [AD1.1.10] (Phycoerythrin) (ab72467)
    and he offered his antibodies to us. However this antibody seems to be used for Flow Cytometry only. How can we use this antibody to show that our solution actually contain his-tagged proteins?

    Read More
    Answer

    Thank you for contacting us. Indeed we have only tested this antibody for Flow Cytometry. You may wish to try an ELISA to test your solution, we have a protocol for ELISA with a fluorophore conjugated primary antibody here:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11421

    We cannot guarantee the antibody will work for this application however. We do have 6X His tag antibodies that have been tested for ELISA such as ab9108.

    Read More

    Question

    Inquiry: Dear Sir/Madam I have a question about the antibodies that I am going to purchase. I am going to purchase a Anti-6X His tag (PE)antibody (ab72467) for my Flow cytometry analysis on bacterial cells (Salmonella Typhimurium). I just want to find the 1. Ab concentration of the product, 2. the F:P ratio, and 3. a suitible isotype control. I've narrow down the isotype control to two possible mAb (PE), which are (ab1280) and (ab91357). Is it possible for these isotype controls to react with Salmonella typhimurium proteins? are they raised to against any bacterial proteins? I's also like to know their Ab concentration and F:P ratio too. Thanks very much

    Read More
    Answer

    In answer to your questions:
    1. Ab concentration of anti-6X His tag (PE), ab72467
    The concentration of this antibody is 69 ug/mL.
    2. The F:P ratio of the antibody
    The F:P ratio of ab72467 is 1:1
    3. A suitable isotype control
    I think either of the antibodies you have suggested would be suitable as an isotype control. The first, ab1280, is supplied at a concentration of 23 ug/mL and the F:P ratio is lot dependent. The current lot is a ratio of 0.9. The second antibody, ab91357, is supplied at a concentration of 7.5 ug/mL and the F:P ratio is between 1-2. It is very unlikely that either of these isotype controls will cross react with your Salmonella typhimurium however as we have not tested this ourselves we would be able to guarantee it.

    Read More

    Question

    I am going to purchase a Anti-6X His tag antibody (ab72467) for my Flow cytometry analysis. I'm just wondering if Abcam also supply other antibodies which can be used for my analysis as an Isotype control (Negative control). Should be something that doesn't react with my Histidine tag, but also has the Phycoerythrin conjugate.

    Read More
    Answer

    I am happy to confirm that we offer a isotype control for ab72467 that is also PE conjugated:

    ab91357

    https://www.abcam.com/PE-Mouse-IgG1-B116-Isotype-Control-ab91357.html (or use the following: https://www.abcam.com/PE-Mouse-IgG1-B116-Isotype-Control-ab91357.html).

    ab91357 was not specifically tested to not bind to HIS tags. But we believe it is very unlikely since the immunogen used for this antibody was a vegetal immunogen.

    Read More

    Question

    I would be grateful if you could inform me the molecular weight if your Anti-6X His tag® antibody, product number ab72467.

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    Answer

    >The molecular weight of the antibody itsself will be ˜150 kDa plus anadditional 240 kDa from the PE fluorophore. The molecular weight detected by the antibody will depend on what protein is His-tagged. The 6X His tag will add < 1 kDa to the weight of the protein, so the band should appear at the same molecular weight as the untagged protein.

    Read More

    Flow Cytometry abreview for Anti-6X His tag® antibody [AD1.1.10] (Phycoerythrin)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Mutiple Myeloma)
    Specification
    Mutiple Myeloma
    Fixation
    no fixation
    Permeabilization
    No
    Gating Strategy
    one cell population
    Read More

    Mr. Etienne Matous

    Verified customer

    Submitted Nov 17 2011

    Question

    Liquid in vial is white colored instead of Pink..

    Read More
    Answer

    After checking the stock of this antibody again, I can assure you that the antibody liquid should be light pink in colour.

    Read More

    Flow Cytometry abreview for Anti-6X His tag® antibody [AD1.1.10] (Phycoerythrin)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (BALB-3T3)
    Specification
    BALB-3T3
    Preparation
    Cell harvesting/tissue preparation method: trypsin/EDTA
    Sample buffer: PBS with 0.1% BSA
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    Forward scatter and side scatter
    Read More

    Abcam user community

    Verified customer

    Submitted May 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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