Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20547] to 6X His tag®
- Suitable for: ICC, Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product namePE Anti-6X His tag® antibody [EPR20547]
See all 6X His tag® primary antibodies
DescriptionPE Rabbit monoclonal [EPR20547] to 6X His tag®
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: ICC, Flow Cytmore details
Synthetic peptide within 6X His tag®. The exact sequence is proprietary.
- ICC: HEK-293T cells transfected with 6X His tag with GFP tag. Flow Cyt: HEK-293T cells transfected with 6X His tag with GFP tag.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS
Concentration information loading...
PurityProtein A purified
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
Our Abpromise guarantee covers the use of ab237338 in the following tested applications.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with 6X His tag with GFP tag labelling 6X His tag with ab237338 at 1/100 (5 µg/ml). The nuclear counterstain was DAPI (Blue).
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with 6X His tag with GFP tag cells labelling 6X His tag with ab237338 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).
Blocking with 10% Goat serum.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237338 has not yet been referenced specifically in any publications.