Recombinant PE Anti-6X His tag® antibody [EPR20547] (ab237338)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20547] to 6X His tag®
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-6X His tag® antibody [EPR20547]
See all 6X His tag® primary antibodies -
Description
PE Rabbit monoclonal [EPR20547] to 6X His tag® -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells transfected with 6X His tag with MYC tag. Flow Cyt (Intra): HEK-293T cells transfected with 6X His tag with MYC tag.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20547 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/500.
|
|
ICC/IF |
1/100.
|
Notes |
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Flow Cyt (Intra)
1/500. |
ICC/IF
1/100. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
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Confocal image showing positive staining in HEK-293T cells transfected with 6X His tag with MYC tag® expression construct.
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Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with 6X His tag with MYC tag with ab237338 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).
Blocking with 10% Goat serum.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab237338 has not yet been referenced specifically in any publications.