Recombinant PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)
![Flow Cytometry - PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)](https://www.abcam.com/ps/products/212/ab212001/Images/ab212001-1-ab212001-Rabbit-monoclonal-EPR15373B-to-ABCE1--C-terminal-R-PE-Human-K562-cells-flow-cytometry.gif "Flow Cytometry - PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
-
Product name
PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
See all ABCE1 primary antibodies -
Description
PE Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human ABCE1 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P61221 -
Positive control
- Flow cyt: K562 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15373(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab212001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
-
Function
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCE family.
Contains 2 4Fe-4S ferredoxin-type domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6059 Human
- Entrez Gene: 24015 Mouse
- Entrez Gene: 361390 Rat
- Omim: 601213 Human
- SwissProt: P61221 Human
- SwissProt: P61222 Mouse
- Unigene: 12013 Human
- Unigene: 5831 Mouse
-
Alternative names
- 2' 5' oligoadenylate binding protein antibody
- 2''-5''-oligoadenylate-binding protein antibody
- 2'5' oligoadenylate binding protein antibody
see all
Images
-
Flow Cytometry - PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)
Overlay histogram showing K562 cells stained with ab212001 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab212001) (1x 106 in 100µl at 1 µg/ml (1/500)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in K562 cells fixed with 80 % methanol (5 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.
-
![PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)](https://www.abcam.com/ps/products/212/ab212001/Images/ab212001-3-benefits-of-recombinant-antibodies.png)
PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab212001)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab212001 has not yet been referenced specifically in any publications.
