Recombinant PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
![Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)](https://www.abcam.com/ps/products/209/ab209306/Images/ab209306-300740-anti-alpha-synuclein-antibody-mjfr1-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [MJFR1] to Alpha-synuclein
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Alpha-synuclein antibody [MJFR1]
See all Alpha-synuclein primary antibodies -
Description
PE Rabbit monoclonal [MJFR1] to Alpha-synuclein -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human, Drosophila melanogaster
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Immunogen
within Human Alpha-synuclein aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P37840 -
Epitope
The epitope was mapped to amino acids 118-123 (VDPDNE). -
Positive control
- Flow Cyt: Neuro 2A (differentiated) cells, HAP1-WT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
MJFR1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab209306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/2500. |
Target
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Function
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
Tissue specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
Involvement in disease
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similarities
Belongs to the synuclein family. -
Domain
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localization
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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Database links
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- Unigene: 21374 Human
- Unigene: 17484 Mouse
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Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
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Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-SNCA knockout cells (red line) stained with ab209306. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209306, 0.1µg/ml dilution) for 30 min at 22°C.
A rabbit monoclonal IgG isotype control antibody (ab209478) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-SNCA knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm)and 586/15 bandpass filter. -
![Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)](https://www.abcam.com/ps/products/209/ab209306/Images/ab209306-261650-anti-alpha-synuclein-antibody-mjfr1-phycoerythrin-flow-cytometry.jpg)
Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)Overlay histogram showing Neuro 2A (differentiated) cells stained with ab209306 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min at 22°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209306, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)](https://www.abcam.com/ps/products/209/ab209306/Images/ab209306-3-benefits-of-recombinant-antibodies.png)
PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
Datasheets and documents
Certificate of Compliance
References (0)
ab209306 has not yet been referenced specifically in any publications.
