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  1. Link

    pe-alpha-synuclein-antibody-mjfr1-ab209306.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)

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Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
  • Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
  • PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [MJFR1] to Alpha-synuclein
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm

You may also be interested in

Protein
Product image
Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

View more associated products

Overview

  • Product name

    PE Anti-Alpha-synuclein antibody [MJFR1]
    See all Alpha-synuclein primary antibodies
  • Description

    PE Rabbit monoclonal [MJFR1] to Alpha-synuclein
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human, Drosophila melanogaster
  • Immunogen

    within Human Alpha-synuclein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P37840

  • Epitope

    The epitope was mapped to amino acids 118-123 (VDPDNE).
  • Positive control

    • Flow Cyt: Neuro 2A (differentiated) cells, HAP1-WT cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    MJFR1
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Synuclein
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau

Associated products

  • Alternative Versions

    • Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
    • Alexa Fluor® 488 Anti-Alpha-synuclein antibody [MJFR1] (ab195025)
    • Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (ab209420)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Recombinant Protein

    • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

Applications

Our Abpromise guarantee covers the use of ab209306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

Target

  • Function

    May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
  • Tissue specificity

    Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
  • Involvement in disease

    Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
    Parkinson disease 1
    Parkinson disease 4
    Dementia Lewy body
  • Sequence similarities

    Belongs to the synuclein family.
  • Domain

    The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
  • Post-translational
    modifications

    Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
    Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
    Ubiquitinated. The predominant conjugate is the diubiquitinated form.
    Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
  • Cellular localization

    Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
  • Target information above from: UniProt accession P37840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6622 Human
    • Entrez Gene: 20617 Mouse
    • Omim: 163890 Human
    • SwissProt: P37840 Human
    • SwissProt: O55042 Mouse
    • Unigene: 21374 Human
    • Unigene: 17484 Mouse
    • Alternative names

      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • SNCA antibody
      • Snca synuclein antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    Images

    • Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
      Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)

      Overlay histogram showing HAP1 wildtype (green line) and HAP1-SNCA knockout cells (red line) stained with ab209306. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209306, 0.1µg/ml dilution) for 30 min at 22°C.

      A rabbit monoclonal IgG isotype control antibody  (ab209478) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-SNCA  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm)and 586/15 bandpass filter.

    • Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
      Flow Cytometry - PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)

      Overlay histogram showing Neuro 2A (differentiated) cells stained with ab209306 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min at 22°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209306, 1/2500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    • PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
      PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab209306? Please let us know so that we can cite the reference in this datasheet.

    ab209306 has not yet been referenced specifically in any publications.

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