Recombinant PE Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225512)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR19342] to Annexin A1/ANXA1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Annexin A1/ANXA1 antibody [EPR19342]
See all Annexin A1/ANXA1 primary antibodies -
Description
PE Rabbit monoclonal [EPR19342] to Annexin A1/ANXA1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Recombinant fragment within Human Annexin A1/ANXA1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04083 -
Positive control
- Flow Cyt (intra): NIH3T3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19342 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab214486)
- Anti-Annexin A1/ANXA1 antibody [EPR19342] - BSA and Azide free (ab222398)
- APC Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225511)
- Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab225513)
- Alexa Fluor® 647 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab309365)
- Alexa Fluor® 555 Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab313664)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt (Intra)
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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Database links
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 25380 Rat
- Omim: 151690 Human
- SwissProt: P04083 Human
- SwissProt: P10107 Mouse
- SwissProt: P07150 Rat
- Unigene: 494173 Human
see all -
Alternative names
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
Images
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Overlay histogram showing NIH3T3 cells stained with ab225512 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225512, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab225512 has been referenced in 3 publications.
- Jarosch S et al. ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples. STAR Protoc 3:101374 (2022). PubMed: 35586313
- Jarosch S et al. Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry. Cell Rep Methods 1:100104 (2021). PubMed: 35475000
- Oshima T et al. Biomarker analysis to predict the pathological response to neoadjuvant chemotherapy in locally advanced gastric cancer: An exploratory biomarker study of COMPASS, a randomized phase II trial. Oncotarget 11:2906-2918 (2020). PubMed: 32774771