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    pe-ass1-antibody-epr12398-ab210451.pdf

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Signal Transduction Metabolism Amino Acids
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant PE Anti-ASS1 antibody [EPR12398] (ab210451)

  • Datasheet
  • Certificate of Compliance
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Immunocytochemistry - PE Anti-ASS1 antibody [EPR12398] (ab210451)
  • Flow Cytometry (Intracellular) - PE Anti-ASS1 antibody [EPR12398] (ab210451)
  • Immunocytochemistry/ Immunofluorescence - PE Anti-ASS1 antibody [EPR12398] (ab210451)
  • PE Anti-ASS1 antibody [EPR12398] (ab210451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR12398] to ASS1
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP Unconjugated

You may also be interested in

Primary
Product image
Anti-IDH3A antibody (ab228596)
Knockout
Product image
Human ASS1 knockout HeLa cell line (ab264989)
Protein
Product image
Recombinant Human ASS1 protein (ab98084)

View more associated products

Overview

  • Product name

    PE Anti-ASS1 antibody [EPR12398]
    See all ASS1 primary antibodies
  • Description

    PE Rabbit monoclonal [EPR12398] to ASS1
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): HeLa cells ICC/IF: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12398
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-ASS1 antibody [EPR12398] (ab170952)
    • Alexa Fluor® 488 Anti-ASS1 antibody [EPR12398] (ab208412)
    • HRP Anti-ASS1 antibody [EPR12398] (ab209018)
    • Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • KO cell lines

    • Human ASS1 knockout HeLa cell line (ab264989)
  • Recombinant Protein

    • Recombinant Human ASS1 protein (ab98084)
  • Related Products

    • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Flow Cyt (Intra)
1/500.
Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Flow Cyt (Intra)
1/500.

Target

  • Pathway

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
  • Involvement in disease

    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
  • Sequence similarities

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Target information above from: UniProt accession P00966 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280726 Cow
    • Entrez Gene: 445 Human
    • Entrez Gene: 11898 Mouse
    • Entrez Gene: 25698 Rat
    • Omim: 603470 Human
    • SwissProt: P14568 Cow
    • SwissProt: P00966 Human
    • SwissProt: P16460 Mouse
    • SwissProt: P09034 Rat
    • Unigene: 160786 Human
    • Unigene: 3217 Mouse
    • Unigene: 5078 Rat
    see all
  • Alternative names

    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

Images

  • Immunocytochemistry - PE Anti-ASS1 antibody [EPR12398] (ab210451)
    Immunocytochemistry - PE Anti-ASS1 antibody [EPR12398] (ab210451)
    ab210451 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab210451 at 1/500 dilution and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Flow Cytometry (Intracellular) - PE Anti-ASS1 antibody [EPR12398] (ab210451)
    Flow Cytometry (Intracellular) - PE Anti-ASS1 antibody [EPR12398] (ab210451)

    Overlay histogram showing HeLa cells stained with ab210451 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210451, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-ASS1 antibody [EPR12398] (ab210451)
    Immunocytochemistry/ Immunofluorescence - PE Anti-ASS1 antibody [EPR12398] (ab210451)

    ab210451 staining ASS1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210451 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • PE Anti-ASS1 antibody [EPR12398] (ab210451)
    PE Anti-ASS1 antibody [EPR12398] (ab210451)

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab210451? Please let us know so that we can cite the reference in this datasheet.

ab210451 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Flow Cytometry abreview for PE Anti-ASS1 antibody [EPR12398]

Good
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (T cell)
Permeabilization
Yes - foxp3 fix perm buffer kit from ebioscience
Gating Strategy
live CD8 T cells were gated
Specification
T cell
Preparation
Cell harvesting/tissue preparation method: T cells taken from mouse spleen
Sample buffer: FACS buffer
Fixation
foxp3 fix perm buffer kit from ebioscience
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 12 2022

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