Recombinant PE Anti-ATF5 antibody [EPR18286] (ab225335)
![Flow Cytometry - PE Anti-ATF5 antibody [EPR18286] (ab225335)](https://www.abcam.com/ps/products/225/ab225335/Images/ab225335-312644-anti-atf5-antibody-epr18286-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-ATF5 antibody [EPR18286] (ab225335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR18286] to ATF5
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-ATF5 antibody [EPR18286]
See all ATF5 primary antibodies -
Description
PE Rabbit monoclonal [EPR18286] to ATF5 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human
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Immunogen
Synthetic peptide within Human ATF5 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y2D1 -
Positive control
- Flow Cyt: NIH3T3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18286 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab225335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Relevance
ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 22809 Human
- Entrez Gene: 107503 Mouse
- Entrez Gene: 282840 Rat
- Omim: 606398 Human
- SwissProt: Q9Y2D1 Human
- SwissProt: O70191 Mouse
- SwissProt: Q6P788 Rat
- Unigene: 9754 Human
see all -
Alternative names
- Activating transcription factor 5 alpha/beta antibody
- Activating transcription factor 5 antibody
- Activating transcription factor X antibody
see all
Images
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Flow Cytometry - PE Anti-ATF5 antibody [EPR18286] (ab225335)
Overlay histogram showing NIH3T3 cells stained with ab225335 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225335, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-ATF5 antibody [EPR18286] (ab225335)](https://www.abcam.com/ps/products/225/ab225335/Images/ab225335-3-benefits-of-recombinant-antibodies.png)
PE Anti-ATF5 antibody [EPR18286] (ab225335)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab225335 has not yet been referenced specifically in any publications.
