RecombinantRabMAb

Recombinant PE Anti-ATG7 antibody [EP1759Y] (ab211584)

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Flow Cytometry - PE Anti-ATG7 antibody [EP1759Y] (ab211584)
  • PE Anti-ATG7 antibody [EP1759Y] (ab211584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP1759Y] to ATG7
  • Suitable for: Flow Cyt
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-ATG7 antibody [EP1759Y]
    See all ATG7 primary antibodies

  • Description

    PE Rabbit monoclonal [EP1759Y] to ATG7

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity


    Predicted to work with: Human

  • Immunogen

    Synthetic peptide within Human ATG7 aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O95352

  • Positive control

    • Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab211584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

  • Tissue specificity

    Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

  • Sequence similarities

    Belongs to the ATG7 family.

  • Domain

    The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
    The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.

  • Post-translational
    modifications

    Acetylated by EP300.

  • Cellular localization

    Cytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
  • Information by UniProt

  • Database links

    • Alternative names

      • 1810013K23Rik antibody
      • Apg 7 antibody
      • APG7 autophagy 7 like antibody
      • APG7 autophagy 7-like (S. cerevisiae) antibody
      • APG7 like antibody
      • APG7, S. cerevisiae, homolog of antibody
      • APG7-like antibody
      • APG7L antibody
      • ATG 7 antibody
      • ATG12-activating enzyme E1 ATG7 antibody
      • ATG7 antibody
      • ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
      • ATG7 autophagy related 7 homolog antibody
      • ATG7_HUMAN antibody
      • Atg7l antibody
      • Autophagy 7, S. cerevisiae, homolog of antibody
      • Autophagy related protein 7 antibody
      • Autophagy-related 7 (yeast) antibody
      • Autophagy-related protein 7 antibody
      • DKFZp434N0735 antibody
      • GSA 7 antibody
      • GSA7 antibody
      • hAGP7 antibody
      • Ubiquitin activating enzyme E1 like protein antibody
      • Ubiquitin-activating enzyme E1-like protein antibody
      • Ubiquitin-like modifier-activating enzyme ATG7 antibody
      see all

    Images

    • Flow Cytometry - PE Anti-ATG7 antibody [EP1759Y] (ab211584)
      Flow Cytometry - PE Anti-ATG7 antibody [EP1759Y] (ab211584)
      Overlay histogram showing HeLa cells stained with ab211584 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211584, 1/5000 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

      This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
    • PE Anti-ATG7 antibody [EP1759Y] (ab211584)
      PE Anti-ATG7 antibody [EP1759Y] (ab211584)

    References (0)

    Publishing research using ab211584? Please let us know so that we can cite the reference in this datasheet.

    ab211584 has not yet been referenced specifically in any publications.

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