PE Anti-beta 2 Microglobulin antibody [B2M-01] (ab49424)

Submit a review Q&A (1)References (2)

Key features and details

  • PE Mouse monoclonal [B2M-01] to beta 2 Microglobulin
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2a

Overview

  • Product name

    PE Anti-beta 2 Microglobulin antibody [B2M-01]
    See all beta 2 Microglobulin primary antibodies

  • Description

    PE Mouse monoclonal [B2M-01] to beta 2 Microglobulin

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    This antibody reacts with beta 2 microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin. Beta 2 mircoglobulin is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog

  • Immunogen

    Full length native protein (purified) (Human)

  • Positive control

    • Peripheral blood lymphocytes.

  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.

  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt

  • Database links

    • Alternative names

      • B2M antibody
      • B2MG_HUMAN antibody
      • Beta 2 microglobin antibody
      • Beta 2 microglobulin antibody
      • Beta 2 microglobulin precursor antibody
      • Beta chain of mhc class 1 proteins antibody
      • Beta chain of MHC class I molecules antibody
      • Beta-2-microglobulin form pI 5.3 antibody
      • CDABP0092 antibody
      • Hdcma22p antibody
      • IMD43 antibody
      see all

    References (2)

    Publishing research using ab49424? Please let us know so that we can cite the reference in this datasheet.

    ab49424 has been referenced in 2 publications.

    • Khurana M  et al. Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol 21:1257-65 (2006). PubMed: 16810512
    • Hilgert I  et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984). PubMed: 6394369

    Customer reviews and Q&As

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    Question

    Zu dem angesprochenen Antikörper „Anti-CD44 antibody [F10-44-2] (PE/Cy7 ®) (ab46793)“ hätte ich noch folgende Fragen. 1.       Bedeutet die Tatsache, dass der Antikörper nicht in  der Liste mit nicht-maus-kreuzreaktiven Antikörpern auftaucht, dass er nicht getestet wurde oder das er getestet wurde und kreuzreaktiv ist? 2.       Der Antikörper ist für die Immunhistologie ausgeschrieben und mich würde interessieren mit welcher Methodik Cy7® detektiert werden kann, da ich es bisher nur aus der Durchflusszytometrie kenne. Zeiss zum Beispiel gibt an kein passendes Filtersystem für Cy7® zu haben. Ich würde mich über eine Antwort freuen.

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    Answer

    Vielen Dank für die Anfrage. Dass ab46793 nicht auf der Liste auftaucht, bedeutet dass er nicht getestet wurde, ob er mit Maus kreuzreagiert. In der Tat ist ab46793 für die Immunhistochemie (IHC-P/Fr - neben anderen Anwendungen wie Durchflusszytometrie) garantiert. Allerdings ist dieser CD44- Antikörper mit dem Tandemkonjugat PE-Cy7 gekoppelt. Welche Anregungswellenlängen beziehungsweise Filter Du für die Detektion benötigst, kannst Du anhand des Spektrums ersehen (Details dazu in Spectra-Viewern von eBioscience http://www.ebioscience.com/resources/fluorplan-spectra-viewer.htm  oder Biolegend http://www.biolegend.com/spectraanalyzer etc.) und beim Hersteller des Mikroskops erfragen. Im Allgemeinen sind PE und Tandem-Fluorochrome wie PE-Cy7 jedoch recht instabil und besitzen eine schwache Fluoreszenz-Intensität, so dass sie in der Regel wenig für die Mikroskopie geeignet sind. Wie auf dem Datenblatt von ab46793 beschrieben, würde ich deswegen für die Detektion dieses CD44- Antikörpers in der IHC einen sekundären Antikörper wie zum Beispiel ab96879 oder ab98697 empfehlen (je nach Fluorochrom-Belegung bei einer Mehrfachfärbung beziehungsweise vorhandenem Filtersystem). Daher ist es eigentlich nicht notwendig, den Klon [F10-44-2] als PE-Cy7-Konjugat zu nehmen (es sei denn, es sind noch andere Experimente damit geplant), sondern Du kannst ihn auch unkonjugiert erwerben (ab6124): ab6124: Click here (or use the following: https://www.abcam.com/CD44-antibody-F10-44-2-ab6124.html). ab96879: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html). ab98697: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-FITC-preadsorbed-ab98697.html). Ich hoffe, diese Informationen helfen Dir weiter. Bitte zögere nicht, Dich wieder bei uns zu melden, falls Du weitere Fragen hast. 

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