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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
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RecombinantRabMAb

Recombinant PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)

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  • Certificate of Compliance
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Flow Cytometry - PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)
  • PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR17923] to c-Myc (phospho T58)
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

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Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Protein
Recombinant human c-Myc protein (Active) (ab169901)

View more associated products

Overview

  • Product name

    PE Anti-c-Myc (phospho T58) antibody [EPR17923]
    See all c-Myc primary antibodies
  • Description

    PE Rabbit monoclonal [EPR17923] to c-Myc (phospho T58)
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human c-Myc aa 50-150 (phospho T58). The exact sequence is proprietary.
    Database link: P01106

  • Positive control

    • Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17923
  • Isotype

    IgG
  • Light chain type

    lambda
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Signal Transduction
    • Antibodies
    • c-myc

Associated products

  • Alternative Versions

    • Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)
    • Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
  • Recombinant Protein

    • Recombinant human c-Myc protein (Active) (ab169901)
  • Related Products

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Applications

Our Abpromise guarantee covers the use of ab237249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

Target

  • Function

    Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in disease

    Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4609 Human
    • Omim: 190080 Human
    • SwissProt: P01106 Human
    • Unigene: 202453 Human
    • Form

      c-Myc is also expressed in the cytoplasm.
    • Alternative names

      • AU016757 antibody
      • Avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c Myc antibody
      • Cellular myelocytomatosis oncogene antibody
      • Class E basic helix-loop-helix protein 39 antibody
      • MGC105490 antibody
      • MRTL antibody
      • Myc antibody
      • Myc protein antibody
      • Myc proto oncogene protein antibody
      • Myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • MYC_HUMAN antibody
      • Myc2 antibody
      • myca antibody
      • MYCC antibody
      • Myelocytomatosis oncogene a antibody
      • Myelocytomatosis oncogene antibody
      • Niard antibody
      • Nird antibody
      • oncogene c-Myc antibody
      • Oncogene Myc antibody
      • OTTHUMP00000158589 antibody
      • OTTHUMP00000227763 antibody
      • Proto-oncogene c-Myc antibody
      • Protooncogene homologous to myelocytomatosis virus antibody
      • RNCMYC antibody
      • Transcription factor p64 antibody
      • Transcriptional regulator Myc-A antibody
      • V-Myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      • zc-myc antibody
      see all

    Images

    • Flow Cytometry - PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)
      Flow Cytometry - PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)

      Overlay histogram showing HeLa cells (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab237249 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS/10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237249) (1x106 in 100 µl at 1:500 (1 µg/ml)) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20 mW Blue laser (488 nm) and 585/42 bandpass filter.

    • PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)
      PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab237249? Please let us know so that we can cite the reference in this datasheet.

    ab237249 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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