PE Anti-C5a-R antibody [20/70] (ab53434)
Key features and details
- PE Rat monoclonal [20/70] to C5a-R
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-C5a-R antibody [20/70]
See all C5a-R primary antibodies -
Description
PE Rat monoclonal [20/70] to C5a-R -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
Blocks the binding of the C5a to murine C5a-R -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse C5a-R. RBL-2H3 transfected cells expressing murine C5aR.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 1 ml distilled water. -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
20/70 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of this concentration to label 10^6 cells in 100ul ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 10ul of this concentration to label 10^6 cells in 100ul ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsSulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12273 Mouse
- SwissProt: P30993 Mouse
- SwissProt: Q3TZ86 Mouse
- Unigene: 247623 Mouse
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Alternative names
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab53434 has been referenced in 2 publications.
- Pantarelli C et al. The GPCR adaptor protein norbin suppresses the neutrophil-mediated immunity of mice to pneumococcal infection. Blood Adv 5:3076-3091 (2021). PubMed: 34402884
- Chen XH et al. Deficiency of Complement C3a and C5a Receptors Prevents Angiotensin II-Induced Hypertension via Regulatory T Cells. Circ Res 122:970-983 (2018). PubMed: 29437833