RecombinantRabMAb

Recombinant PE Anti-Caldesmon/CDM antibody [E89] (ab211580)

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Immunocytochemistry/ Immunofluorescence - PE Anti-Caldesmon/CDM antibody [E89] (ab211580)
  • Flow Cytometry - PE Anti-Caldesmon/CDM antibody [E89] (ab211580)
  • PE Anti-Caldesmon/CDM antibody [E89] (ab211580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [E89] to Caldesmon/CDM
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-Caldesmon/CDM antibody [E89]
    See all Caldesmon/CDM primary antibodies

  • Description

    PE Rabbit monoclonal [E89] to Caldesmon/CDM

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    within Human Caldesmon/CDM aa 750 to the C-terminus (phospho S789). The exact sequence is proprietary.
    Database link: Q05682

  • Positive control

    • Flow Cytometry: HeLa cells ICC/IF: HeLa cells

  • General notes

     

     

     This product was previously labelled as Caldesmon

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab211580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.

  • Tissue specificity

    High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

  • Sequence similarities

    Belongs to the caldesmon family.

  • Domain

    The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.

  • Post-translational
    modifications

    In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CAD antibody
    • CALD 1 antibody
    • CALD1 antibody
    • CALD1_HUMAN antibody
    • Caldesmon 1 antibody
    • Caldesmon 1 Isoform 1 antibody
    • Caldesmon 1 Isoform 2 antibody
    • Caldesmon 1 Isoform 3 antibody
    • Caldesmon 1 Isoform 4 antibody
    • Caldesmon 1 Isoform 5 antibody
    • Caldesmon antibody
    • Caldesmon1 antibody
    • CDM antibody
    • H CAD antibody
    • HCAD antibody
    • L CAD antibody
    • LCAD antibody
    • MGC21352 antibody
    • NAG22 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - PE Anti-Caldesmon/CDM antibody [E89] (ab211580)
    Immunocytochemistry/ Immunofluorescence - PE Anti-Caldesmon/CDM antibody [E89] (ab211580)

    Ab211580 staining Caldesmon/CDM in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211580 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - PE Anti-Caldesmon/CDM antibody [E89] (ab211580)
    Flow Cytometry - PE Anti-Caldesmon/CDM antibody [E89] (ab211580)

    Overlay histogram showing HeLa cells stained with ab211580 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211580, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • PE Anti-Caldesmon/CDM antibody [E89] (ab211580)
    PE Anti-Caldesmon/CDM antibody [E89] (ab211580)

References (0)

Publishing research using ab211580? Please let us know so that we can cite the reference in this datasheet.

ab211580 has not yet been referenced specifically in any publications.

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