Key features and details
- PE Mouse monoclonal [FMC 75] to Calreticulin
- Suitable for: Flow Cyt
- Reacts with: Human, Monkey
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-Calreticulin antibody [FMC 75]
See all Calreticulin primary antibodies
DescriptionPE Mouse monoclonal [FMC 75] to Calreticulin
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human, Monkey
Recombinant full length protein corresponding to Calreticulin.
- Jurkat cells.
This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityTissue culture supernatant
Clone numberFMC 75
Light chain typekappa
Our Abpromise guarantee covers the use of ab83220 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Sequence similaritiesBelongs to the calreticulin family.
DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
ab83220 has been referenced in 6 publications.
- Huang Y et al. Comparison of the effects of photon, proton and carbon-ion radiation on the ecto-calreticulin exposure in various tumor cell lines. Ann Transl Med 7:542 (2019). PubMed: 31807524
- Vera CA et al. The nerve growth factor alters calreticulin translocation from the endoplasmic reticulum to the cell surface and its signaling pathway in epithelial ovarian cancer cells. Int J Oncol 50:1261-1270 (2017). PubMed: 28260038
- Thierry S et al. The DNA Repair Inhibitor Dbait Is Specific for Malignant Hematologic Cells in Blood. Mol Cancer Ther 16:2817-2827 (2017). PubMed: 28947503
- Draganov D et al. Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via Ivermectin induces a non-apoptotic and inflammatory form of cancer cell death. Sci Rep 5:16222 (2015). PubMed: 26552848
- Angelova AL et al. Complementary induction of immunogenic cell death by oncolytic parvovirus H-1PV and gemcitabine in pancreatic cancer. J Virol 88:5263-76 (2014). Flow Cyt ; Human . PubMed: 24574398
- Wei H et al. Bovine Herpesvirus Type 1 (BHV-1) U(L)49.5 Luminal Domain Residues 30 to 32 Are Critical for MHC-I Down-Regulation in Virus-Infected Cells. PLoS One 6:e25742 (2011). Flow Cyt ; Cow . PubMed: 22046246