Recombinant PE Anti-CCR2 antibody [EPR20844] (ab245899)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20844] to CCR2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-CCR2 antibody [EPR20844]
See all CCR2 primary antibodies -
Description
PE Rabbit monoclonal [EPR20844] to CCR2 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: C57BL/6 mouse splenocytes.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20844 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CCR2 antibody [EPR20844] (ab216863)
- Anti-CCR2 antibody [EPR20844] - BSA and Azide free (ab233701)
- Alexa Fluor® 488 Anti-CCR2 antibody [EPR20844] (ab245896)
- Alexa Fluor® 647 Anti-CCR2 antibody [EPR20844] (ab245897)
- APC Anti-CCR2 antibody [EPR20844] (ab245898)
- Alexa Fluor® 594 Anti-CCR2 antibody [EPR20844] (ab286808)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt
1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
- Unigene: 6272 Mouse
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Alternative names
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
Images
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C57BL/6 mouse splenocytes stained with ab245899 (right) or Rabbit IgG (monoclonal) Phycoerythrin (Isotype control (ab209478)) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab245899) or Rabbit IgG (monoclonal) Phycoerythrin (Isotype control (ab209478) (1x 106 cells in 100 µl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with Ly6G/GR1 PerCP conjugated antibody (ab236728).
Acquisition of >30,000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 586/15 bandpass filter.
Events were gated on viable lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab245899 has not yet been referenced specifically in any publications.