Key features and details
- PE Rabbit monoclonal [EPR20844] to CCR2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product namePE Anti-CCR2 antibody [EPR20844]
See all CCR2 primary antibodies
DescriptionPE Rabbit monoclonal [EPR20844] to CCR2
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse CCR2 aa 1-100. The exact sequence is proprietary.
Database link: P51683
- Flow Cyt: C57BL/6 mouse splenocytes.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab245899 in the following tested applications.
The cellular localisation of this product has been verified in ICC/IF.
FunctionReceptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
C57BL/6 mouse splenocytes stained with ab245899 (right) or Rabbit IgG (monoclonal) Phycoerythrin (Isotype control (ab209478)) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab245899) or Rabbit IgG (monoclonal) Phycoerythrin (Isotype control (ab209478) (1x 106 cells in 100 µl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with Ly6G/GR1 PerCP conjugated antibody (ab236728).
Acquisition of >30,000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 586/15 bandpass filter.
Events were gated on viable lymphocytes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245899 has not yet been referenced specifically in any publications.