Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR22191] to CCR2
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product namePE Anti-CCR2 antibody [EPR22191]
See all CCR2 primary antibodies
DescriptionPE Rabbit monoclonal [EPR22191] to CCR2
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- Flow Cyt: Human PBMCs.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab270167 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10µg/mL human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab270167) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 µl at 1µg/mL (1/500)) for 30 min on ice. The cells were simultaneously stained with CD14.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live single cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270167 has not yet been referenced specifically in any publications.