PE Anti-CCR7 antibody [4B12] (ab95669)
![Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)](https://www.abcam.com/ps/products/95/ab95669/Images/ab95669-109930-anti-ccr7-antibody-4b12-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)")
Key features and details
- PE Rat monoclonal [4B12] to CCR7
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-CCR7 antibody [4B12]
See all CCR7 primary antibodies -
Description
PE Rat monoclonal [4B12] to CCR7 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab95669 stains Mouse CCR7-GFP fusion protein-transfected RBL cells -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CCR7. CCR7 Rat basophilic leukaemia (RBL)-2H3 transfectants.
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Positive control
- C57BL/6, Balb/C and SJL/J splenocytes and thymocytes.
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General notes
Excitation: 488 nm Emission: 575 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4B12 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1-5µg for 106 cells.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 1-5µg for 106 cells. ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
Tissue specificity
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12775 Mouse
- SwissProt: P47774 Mouse
- Unigene: 2932 Mouse
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Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
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Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)Staining of C57BL/6 splenocytes with Anti-Mouse CD4 FITC and 0.5 µg of ab95669 with staining carried out at 4 °C (left) or 37 °C (right). Cells in the lymphocyte gate were used for analysis
Datasheets and documents
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Datasheet download
References (1)
ab95669 has been referenced in 1 publication.
- Waldner H et al. The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells. J Immunol 176:5455-62 (2006). PubMed: 16622013
