PE Anti-CCR7 antibody [4B12] (ab95669)
![Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)](https://www.abcam.com/ps/products/95/ab95669/Images/ab95669-109930-anti-ccr7-antibody-4b12-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)")
Key features and details
- PE Rat monoclonal [4B12] to CCR7
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-CCR7 antibody [4B12]
See all CCR7 primary antibodies -
Description
PE Rat monoclonal [4B12] to CCR7 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab95669 stains Mouse CCR7-GFP fusion protein-transfected RBL cells -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
CCR7 Rat basophilic leukaemia (RBL)-2H3 transfectants. Spleen cells from immunised Lou/c Rats were fused with cells of the Mouse X63.Ag8.653 myeloma cell line.
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Positive control
- C57BL/6, Balb/C and SJL/J splenocytes and thymocytes.
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General notes
Excitation: 488 nm Emission: 575 nm.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4B12 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab95669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1-5µg for 106 cells. ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
Tissue specificity
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12775 Mouse
- SwissProt: P47774 Mouse
- Unigene: 2932 Mouse
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Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
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Flow Cytometry - PE Anti-CCR7 antibody [4B12] (ab95669)Staining of C57BL/6 splenocytes with Anti-Mouse CD4 FITC and 0.5 µg of ab95669 with staining carried out at 4 °C (left) or 37 °C (right). Cells in the lymphocyte gate were used for analysis
Datasheets and documents
References (1)
ab95669 has been referenced in 1 publication.
- Waldner H et al. The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells. J Immunol 176:5455-62 (2006). PubMed: 16622013
