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  1. Link

    pe-cd105-antibody-mem-229-ab53321.pdf

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Immunology Cell Type Markers CD Endothelial Cells
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PE Anti-CD105 antibody [MEM-229] (ab53321)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (13)

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Abpromise

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Immunohistochemistry (Frozen sections) - PE Anti-CD105 antibody [MEM-229] (ab53321)

    Key features and details

    • PE Mouse monoclonal [MEM-229] to CD105
    • Suitable for: IHC-Fr
    • Reacts with: Human, Pig
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

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    Overview

    • Product name

      PE Anti-CD105 antibody [MEM-229]
      See all CD105 primary antibodies
    • Description

      PE Mouse monoclonal [MEM-229] to CD105
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: IHC-Frmore details
    • Species reactivity

      Reacts with: Human, Pig
    • Immunogen

      Recombinant vaccinia virus containing human CD105 (L-isoform) cDNA

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      MEM-229
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Endothelial Cells
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Cancer
      • Tumor biomarkers
      • Other
      • Cardiovascular
      • Vasculature
      • Endothelium
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Alternative Versions

      • PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)
      • FITC Anti-CD105 antibody [MEM-229] (ab53318)
      • Anti-CD105 antibody [MEM-229] (ab69772)
    • Isotype control

      • PE Mouse IgG2a [X5563] - Isotype Control (ab91363)
    • Recombinant Protein

      • Recombinant human CD105 protein (ab54338)

    Applications

    Our Abpromise guarantee covers the use of ab53321 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr 1/200. Fix with acetone.

    Target

    • Function

      Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
    • Tissue specificity

      Endoglin is restricted to endothelial cells in all tissues except bone marrow.
    • Involvement in disease

      Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P17813 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2022 Human
      • Omim: 131195 Human
      • SwissProt: P17813 Human
      • Unigene: 76753 Human
      • Alternative names

        • AI528660 antibody
        • AI662476 antibody
        • CD 105 antibody
        • CD105 antibody
        • CD105 antigen antibody
        • EGLN_HUMAN antibody
        • END antibody
        • Endoglin antibody
        • Eng antibody
        • FLJ41744 antibody
        • HHT1 antibody
        • ORW antibody
        • ORW1 antibody
        • Osler Rendu Weber syndrome 1 antibody
        • RP11 228B15.2 antibody
        • S endoglin antibody
        • S-endoglin antibody
        • SN6 antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - PE Anti-CD105 antibody [MEM-229] (ab53321)
        Immunohistochemistry (Frozen sections) - PE Anti-CD105 antibody [MEM-229] (ab53321)
        ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (13)

      Publishing research using ab53321? Please let us know so that we can cite the reference in this datasheet.

      ab53321 has been referenced in 13 publications.

      • Queckbörner S  et al. Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile. Stem Cell Res Ther 11:15 (2020). PubMed: 31907034
      • Conley SM  et al. Human Obesity Induces Dysfunction and Early Senescence in Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells. Front Cell Dev Biol 8:197 (2020). PubMed: 32274385
      • Karakas N  et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
      • Borghesi J  et al. Canine amniotic membrane mesenchymal stromal/stem cells: Isolation, characterization and differentiation. Tissue Cell 58:99-106 (2019). PubMed: 31133253
      • Burmeister DM  et al. Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol-Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns. Stem Cells Transl Med 7:360-372 (2018). ICC/IF ; Pig . PubMed: 29457376
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      Can you please send me a list of all of the antibodies you have validated for use in Flow Cytometry on sheep cells?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 17 2012

      Answer

      Thank you for your phone call.

      Attached please find the list of our antibodies that have been validated for use in Flow Cytometry on sheep cells.

      If you are interested in testing one of our other antibodies for use in sheep or Flow Cytometry, I can offer a discount off a future purchase if you buy the antibody now, test it insheep or Flow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me knowwhich antibody you wouldlike to test. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

      2. Purchasethe untested antibodyeither by phone, fax, or online (www.abcam.com).

      3. Test it in sheep or Flow Cytometry.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for sheep or Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Feb 17 2012

      Question

      What is the concentration of lot GR37350?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 26 2012

      Answer

      Thank you for contacting us.

      The IgG concentration of lot GR37350 is 60ug/ml.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jan 26 2012

      Question

      Greetings! I have purchased this antibody and I need to know what the concentration is please. I am conducting multiple-antibody flow cytometry and I need to balance the antibody concentrations, but there is no concentration information on this product, just number of "tests". Thank you!

      Read More

      Abcam community

      Verified customer

      Asked on Jan 05 2009

      Answer

      Thank you for your enquiry. I am afraid that according to the supplier of the product, the exact concentration for this product is proprietary and can not be released. The only information they will provide is the number of tests and dilution factor to use. I know that this is not much information for you to get by with and it would be difficult to standardize your experiments. I can only suggest to try callibrating the amount of antibody used with the intensity of the staining, and compare with other antibodies. I am sorry I can't be of more assistance in this occasion, but if there is anything else that I can help you with, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Jan 05 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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