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PE Anti-CD105 antibody [MEM-229] (ab53321)

Submit a review Q&A (3)References (17)
Immunohistochemistry (Frozen sections) - PE Anti-CD105 antibody [MEM-229] (ab53321)

    Key features and details

    • PE Mouse monoclonal [MEM-229] to CD105
    • Suitable for: IHC-Fr
    • Reacts with: Pig
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    FITC PerCP/Cy5.5® Unconjugated

    Overview

    • Product name

      PE Anti-CD105 antibody [MEM-229]
      See all CD105 primary antibodies

    • Description

      PE Mouse monoclonal [MEM-229] to CD105

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: IHC-Frmore details

    • Species reactivity

      Reacts with: Pig

    • Immunogen

      Recombinant vaccinia virus containing human CD105 (L-isoform) cDNA

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab53321 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    1/200. Fix with acetone.
    Notes
    IHC-Fr
    1/200. Fix with acetone.

    Target

    • Function

      Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.

    • Tissue specificity

      Endoglin is restricted to endothelial cells in all tissues except bone marrow.

    • Involvement in disease

      Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - PE Anti-CD105 antibody [MEM-229] (ab53321)
      Immunohistochemistry (Frozen sections) - PE Anti-CD105 antibody [MEM-229] (ab53321)
      ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.

    References (17)

    Publishing research using ab53321? Please let us know so that we can cite the reference in this datasheet.

    ab53321 has been referenced in 17 publications.

    • Wang J  et al. Osteoclastic effects of mBMMSCs under compressive pressure during orthodontic tooth movement. Stem Cell Res Ther 12:148 (2021). PubMed: 33632323
    • Isik B  et al. Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosis. Stem Cell Res Ther 12:240 (2021). PubMed: 33853680
    • Queckbörner S  et al. Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile. Stem Cell Res Ther 11:15 (2020). PubMed: 31907034
    • Conley SM  et al. Human Obesity Induces Dysfunction and Early Senescence in Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells. Front Cell Dev Biol 8:197 (2020). PubMed: 32274385
    • Karakas N  et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Can you please send me a list of all of the antibodies you have validated for use in Flow Cytometry on sheep cells?

    Read More
    Answer

    Thank you for your phone call.

    Attached please find the list of our antibodies that have been validated for use in Flow Cytometry on sheep cells.

    If you are interested in testing one of our other antibodies for use in sheep or Flow Cytometry, I can offer a discount off a future purchase if you buy the antibody now, test it insheep or Flow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me knowwhich antibody you wouldlike to test. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchasethe untested antibodyeither by phone, fax, or online (www.abcam.com).

    3. Test it in sheep or Flow Cytometry.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for sheep or Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    What is the concentration of lot GR37350?

    Read More
    Answer

    Thank you for contacting us.

    The IgG concentration of lot GR37350 is 60ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Greetings! I have purchased this antibody and I need to know what the concentration is please. I am conducting multiple-antibody flow cytometry and I need to balance the antibody concentrations, but there is no concentration information on this product, just number of "tests". Thank you!

    Read More
    Answer

    Thank you for your enquiry. I am afraid that according to the supplier of the product, the exact concentration for this product is proprietary and can not be released. The only information they will provide is the number of tests and dilution factor to use. I know that this is not much information for you to get by with and it would be difficult to standardize your experiments. I can only suggest to try callibrating the amount of antibody used with the intensity of the staining, and compare with other antibodies. I am sorry I can't be of more assistance in this occasion, but if there is anything else that I can help you with, please do not hesitate to contact me.

    Read More

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