PE Anti-CD146 antibody [P1H12] (ab234280)

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Flow Cytometry - PE Anti-CD146 antibody [P1H12] (ab234280)

    Key features and details

    • PE Mouse monoclonal [P1H12] to CD146
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-CD146 antibody [P1H12]
      See all CD146 primary antibodies

    • Description

      PE Mouse monoclonal [P1H12] to CD146

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rabbit, DogDoes not react with: Rat

    • Immunogen

      Tissue, cells or virus corresponding to Human CD146. Cultured human umbilical cells.

    • Positive control

      • Flow Cyt: HUVEC cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab234280 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    Target

    • Function

      Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

    • Tissue specificity

      Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • A32 antigen antibody
        • CD 146 antibody
        • CD146 antibody
        • CD146 antigen antibody
        • Cell surface glycoprotein MUC18 antibody
        • Cell surface glycoprotein P1H12 antibody
        • Gicerin antibody
        • Mcam antibody
        • Melanoma adhesion molecule antibody
        • Melanoma associated antigen A32 antibody
        • Melanoma associated antigen MUC18 antibody
        • Melanoma associated glycoprotein MUC18 antibody
        • Melanoma cell adhesion molecule antibody
        • Melanoma-associated antigen A32 antibody
        • Melanoma-associated antigen MUC18 antibody
        • MelCAM antibody
        • MUC 18 antibody
        • MUC18 antibody
        • MUC18_HUMAN antibody
        • S endo 1 antibody
        • S endo 1 endothelial associated antigen antibody
        • S-endo 1 endothelial-associated antigen antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD146 antibody [P1H12] (ab234280)
        Flow Cytometry - PE Anti-CD146 antibody [P1H12] (ab234280)

        HUVEC cells. Gated on singlets.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab234280? Please let us know so that we can cite the reference in this datasheet.

      ab234280 has not yet been referenced specifically in any publications.

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