PE Anti-CD151 antibody [50-6] (ab253266)
![Flow Cytometry - PE Anti-CD151 antibody [50-6] (ab253266)](https://www.abcam.com/ps/products/253/ab253266/Images/ab253266-343725-AntiCD151-antibody-506-Phycoerythrin-human-peripheral-blood.jpg "Flow Cytometry - PE Anti-CD151 antibody [50-6] (ab253266)")
Key features and details
- PE Mouse monoclonal [50-6] to CD151
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD151 antibody [50-6]
See all CD151 primary antibodies -
Description
PE Mouse monoclonal [50-6] to CD151 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD151. Human epidermoid carcinoma cell line Hep-3.
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Positive control
- Flow Cyt: Human peripheral blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
50-6 -
Isotype
IgG1 -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab253266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Or 100 µl of whole blood. |
Target
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Function
Essential for the proper assembly of the glomerular and tubular basement membranes in kidney. -
Tissue specificity
Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. -
Involvement in disease
Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 977 Human
- Omim: 602243 Human
- SwissProt: P48509 Human
- Unigene: 654379 Human
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Alternative names
- CD151 antibody
- CD151 antigen antibody
- CD151 molecule (Raph blood group) antibody
see all
Images
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Flow Cytometry - PE Anti-CD151 antibody [50-6] (ab253266)
Flow Cytometry analysis of human peripheral blood labeling CD151 with ab253266. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253266 has not yet been referenced specifically in any publications.
