Key features and details
- PE Mouse monoclonal [3G8] to CD16
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-CD16 antibody [3G8]
See all CD16 primary antibodies
DescriptionPE Mouse monoclonal [3G8] to CD16
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
- Flow Cyt: Lymphocytes from human blood.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA
Concentration information loading...
- PerCP Anti-CD16 antibody [3G8] (ab130761)
- PerCP/Cy5.5® Anti-CD16 antibody [3G8], prediluted (ab157322)
- Anti-CD16 antibody [3G8] - Low endotoxin, Azide free (ab176528)
- Alexa Fluor® 488 Anti-CD16 antibody [3G8] (ab187585)
- APC/Cy7® Anti-CD16 antibody [3G8], prediluted (ab197141)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab234209)
- FITC Anti-CD16 antibody [3G8] (ab239254)
- Alexa Fluor® 647 Anti-CD16 antibody [3G8] (ab239275)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab267386)
Our Abpromise guarantee covers the use of ab117117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
FunctionReceptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificityExpressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage.
Cellular localizationCell membrane. Secreted. Exists also as a soluble receptor.
- Information by UniProt
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
Flow cytometry multicolor surface staining pattern of human lymphocytes using ab117117 at (20 µL reagent/100 µL of peripheral whole blood) and anti-human CD3 APC antibody at (10 µL reagent/100 µL of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using ab117117 at (20 µL reagent/100 µL of peripheral whole blood).
Separation of human CD3 negative CD16 positive NK cells (red-filled) from human CD3 positive CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab117117 at (20 µL reagent/100 µL of peripheral whole blood).
ab117117 has been referenced in 1 publication.
- Congy-Jolivet N et al. Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res 68:976-80 (2008). PubMed: 18281470