PE Anti-CD16 antibody [3G8] (ab117117)
![Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)](https://www.abcam.com/ps/products/117/ab117117/Images/ab117117-2-anti-cd16-antibody-flow-cytometry-lymphocytes-human.jpg "Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)")
Key features and details
- PE Mouse monoclonal [3G8] to CD16
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD16 antibody [3G8]
See all CD16 primary antibodies -
Description
PE Mouse monoclonal [3G8] to CD16 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human neutrophils.
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Positive control
- Flow Cyt: Lymphocytes from human blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
3G8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD16 antibody [3G8] (ab130761)
- PerCP/Cy5.5® Anti-CD16 antibody [3G8], prediluted (ab157322)
- Anti-CD16 antibody [3G8] - Low endotoxin, Azide free (ab176528)
- Alexa Fluor® 488 Anti-CD16 antibody [3G8] (ab187585)
- APC/Cy7® Anti-CD16 antibody [3G8], prediluted (ab197141)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab234209)
- FITC Anti-CD16 antibody [3G8] (ab239254)
- Alexa Fluor® 647 Anti-CD16 antibody [3G8] (ab239275)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab267386)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab117117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- Omim: 146740 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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Alternative names
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
Images
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Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)Flow cytometry multicolor surface staining pattern of human lymphocytes using ab117117 at (20 µL reagent/100 µL of peripheral whole blood) and anti-human CD3 APC antibody at (10 µL reagent/100 µL of peripheral whole blood).
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![Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)](https://www.abcam.com/ps/products/117/ab117117/Images/ab117117-1-anti-cd16-antibody-flow-cytometry-peripheral-whole-blood-human.jpg)
Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)Flow cytometry surface staining pattern of human peripheral whole blood stained using ab117117 at (20 µL reagent/100 µL of peripheral whole blood). -
![Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)](https://www.abcam.com/ps/products/117/ab117117/Images/ab117117-3-anti-cd16-antibody-flow-cytometry-nk-cells-human.jpg)
Flow Cytometry - PE Anti-CD16 antibody [3G8] (ab117117)Separation of human CD3 negative CD16 positive NK cells (red-filled) from human CD3 positive CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab117117 at (20 µL reagent/100 µL of peripheral whole blood).
References (1)
ab117117 has been referenced in 1 publication.
- Congy-Jolivet N et al. Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res 68:976-80 (2008). PubMed: 18281470
