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PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)

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Flow Cytometry - PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)
  • Flow Cytometry - PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)

Key features and details

  • PE Mouse monoclonal [MEM-166] to CD177, prediluted
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-CD177 antibody [MEM-166], prediluted
    See all CD177 primary antibodies

  • Description

    PE Mouse monoclonal [MEM-166] to CD177, prediluted

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates

  • Immunogen

    Tissue, cells or virus corresponding to Human CD177. Human granulocytes

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Size exclusion

  • Purification notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

  • Clonality

    Monoclonal

  • Clone number

    MEM-166

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

Use 20 μl per 100 μl of whole blood or 106 cells in a suspension
Notes
Flow Cyt
Use at an assay dependent concentration.

Use 20 μl per 100 μl of whole blood or 106 cells in a suspension

Target

  • Tissue specificity

    Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).

  • Sequence similarities

    Contains 2 UPAR/Ly6 domains.

  • Post-translational
    modifications

    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD177 antibody
      • CD177 antigen antibody
      • CD177 molecule antibody
      • CD177_HUMAN antibody
      • HNA-2a antibody
      • HNA2A antibody
      • Human neutrophil alloantigen 2a antibody
      • NB1 antibody
      • NB1 glycoprotein antibody
      • NB1 GP antibody
      • Polycythemia rubra vera 1 antibody
      • Polycythemia rubra vera protein 1 antibody
      • PRV-1 antibody
      • PRV1 antibody
      see all

    Images

    • Flow Cytometry - PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)
      Flow Cytometry - PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)
      Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab69777 (20 μl reagent / 100 μl of peripheral whole blood).
       
    • Flow Cytometry - PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)
      Flow Cytometry - PE Anti-CD177 antibody [MEM-166], prediluted (ab69777)

      Flow cytometry surface staining pattern of human peripheral whole blood stained using ab69777 (20 μl reagent / 100 μl of peripheral whole blood).

    References (1)

    Publishing research using ab69777? Please let us know so that we can cite the reference in this datasheet.

    ab69777 has been referenced in 1 publication.

    • Amirbeagi F  et al. Olfactomedin-4 autoantibodies give unusual c-ANCA staining patterns with reactivity to a subpopulation of neutrophils. J Leukoc Biol 97:181-9 (2015). PubMed: 25387833

    Customer reviews and Q&As

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