PE Anti-CD20 antibody [2H7] (ab112461)
![Flow Cytometry - PE Anti-CD20 antibody [2H7] (ab112461)](https://www.abcam.com/ps/products/112/ab112461/Images/ab112461-277219-anti-cd20-antibody-2h7-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD20 antibody [2H7] (ab112461)")
Key features and details
- PE Mouse monoclonal [2H7] to CD20
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-CD20 antibody [2H7]
See all CD20 primary antibodies -
Description
PE Mouse monoclonal [2H7] to CD20 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
Human tonsillar B cells.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes/
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.88% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
2H7 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 105-8 cells.
Use 5µl in a final volume of 100µl. ab91532 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 5µl for 105-8 cells. Use 5µl in a final volume of 100µl. ab91532 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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Flow Cytometry - PE Anti-CD20 antibody [2H7] (ab112461)
Human peripheral blood lymphocytes stained with 5µL (0.06µg) ab112461 (solid line) or 0.06µg PE-conjugated mouse IgG2b isotype control (dashed line).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab112461 has not yet been referenced specifically in any publications.