PE Anti-CD200 / OX2 antibody [MRC OX-2] (ab256289)
![Flow Cytometry - PE Anti-CD200 / OX2 antibody [MRC OX-2] (ab256289)](https://www.abcam.com/ps/products/256/ab256289/Images/ab256289-1-MousemonoclonaltoCD200-Lewisratsplenocytesflowcytometry.jpg "Flow Cytometry - PE Anti-CD200 / OX2 antibody [MRC OX-2] (ab256289)")
Key features and details
- PE Mouse monoclonal [MRC OX-2] to CD200 / OX2
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD200 / OX2 antibody [MRC OX-2]
See all CD200 / OX2 primary antibodies -
Description
PE Mouse monoclonal [MRC OX-2] to CD200 / OX2 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/2500.
|
| Notes |
|---|
|
Flow Cyt
1/2500. |
Target
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Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24560 Rat
- SwissProt: P04218 Rat
- Unigene: 7085 Rat
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Alternative names
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
Images
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Flow Cytometry - PE Anti-CD200 / OX2 antibody [MRC OX-2] (ab256289)
Lewis rat splenocytes stained with ab256289 (right) or mouse IgG1κ Phycoerythrin (ab91357) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (abLewis rat splenocytes) or mouse IgG1κ Phycoerythrin (ab91357) isotype (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. Events were gated on live lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab256289 has not yet been referenced specifically in any publications.
