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  1. Link

    pe-cd200--ox2-antibody-mrc-ox90-ab33739.pdf

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Immunology Adaptive Immunity T Cells CD
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PE Anti-CD200 / OX2 antibody [MRC OX90] (ab33739)

  • Datasheet
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Flow Cytometry - PE Anti-CD200 / OX2 antibody [MRC OX90] (ab33739)

    Key features and details

    • PE Rat monoclonal [MRC OX90] to CD200 / OX2
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Product image
    Recombinant Human CD200 / OX2 protein (ab219476)

    View more associated products

    Overview

    • Product name

      PE Anti-CD200 / OX2 antibody [MRC OX90]
      See all CD200 / OX2 primary antibodies
    • Description

      PE Rat monoclonal [MRC OX90] to CD200 / OX2
    • Host species

      Rat
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Specificity

      Recognises the mouse CD200 cell surface antigen, also known as OX2.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Mouse CD200- Rat CD4 fusion protein

    • Positive control

      • CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.
    • General notes

      Purified IgG conjugated to R. Phycoerythrin (RPE).

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      MRC OX90
    • Myeloma

      Y3/Ag1.2.3
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity

    Associated products

    • Alternative Versions

      • Anti-CD200 / OX2 antibody [MRC OX90] - BSA and Azide free (ab244564)
      • Anti-CD200 / OX2 antibody [MRC OX90] (ab33734)
      • Biotin Anti-CD200 / OX2 antibody [MRC OX90] (ab33735)
      • FITC Anti-CD200 / OX2 antibody [MRC OX90] (ab33737)
    • Recombinant Protein

      • Recombinant Human CD200 / OX2 protein (ab219476)

    Applications

    Our Abpromise guarantee covers the use of ab33739 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P41217 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 17470 Mouse
      • SwissProt: O54901 Mouse
      • Unigene: 245851 Mouse
      • Alternative names

        • Antigen identified by monoclonal antibody MRC OX 2 antibody
        • CD200 antibody
        • CD200 antigen antibody
        • CD200 molecule antibody
        • MOX1 antibody
        • MOX2 antibody
        • MRC antibody
        • MRC OX 2 antigen antibody
        • My033 antibody
        • OX 2 antibody
        • OX 2 membrane glycoprotein precursor antibody
        • OX-2 membrane glycoprotein antibody
        • OX2G antibody
        • OX2G_HUMAN antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD200 / OX2 antibody [MRC OX90] (ab33739)
        Flow Cytometry - PE Anti-CD200 / OX2 antibody [MRC OX90] (ab33739)
        ab33739 staining of mouse splenocytes.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab33739? Please let us know so that we can cite the reference in this datasheet.

      ab33739 has been referenced in 1 publication.

      • Zhou Y  et al. Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes. Neoplasia 20:1059-1069 (2018). PubMed: 30227305

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab33739.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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