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  1. Link

    pe-cd200r-antibody-ox102-ab34135.pdf

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Immunology Adaptive Immunity T Cells CD
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PE Anti-CD200R antibody [OX102] (ab34135)

  • Datasheet
Reviews (4) Submit a question References (1)

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Flow Cytometry - PE Anti-CD200R antibody [OX102] (ab34135)

    Key features and details

    • PE Mouse monoclonal [OX102] to CD200R
    • Reacts with: Rat
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Recombinant Human CD200R protein (ab151347)

    View more associated products

    Overview

    • Product name

      PE Anti-CD200R antibody [OX102]
      See all CD200R primary antibodies
    • Description

      PE Mouse monoclonal [OX102] to CD200R
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Specificity

      This antibody is specific for the CD200R ligand.
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Tissue/ cell preparation: Membrane fraction of thioglycollate elicited rat peripheral cells.

    • General notes


      This antibody has been shown to block the interaction of OX2 ligand with CD200.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      This antibody was purified tissue culture supernatant.
    • Primary antibody notes

      This antibody has been shown to block the interaction of OX2 ligand with CD200.
    • Clonality

      Monoclonal
    • Clone number

      OX102
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity

    Associated products

    • Alternative Versions

      • Anti-CD200R antibody [OX102] (ab243838)
      • Anti-CD200R antibody [OX102] - BSA and Azide free (ab244592)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)
    • Recombinant Protein

      • Recombinant Human CD200R protein (ab151347)

    Target

    • Function

      Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
    • Tissue specificity

      Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
    • Sequence similarities

      Belongs to the CD200R family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q8TD46 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 64357 Rat
      • SwissProt: Q9ES58 Rat
      • Unigene: 124439 Rat
      • Alternative names

        • CD 200R antibody
        • CD200 cell surface glycoprotein receptor antibody
        • CD200 receptor 1 antibody
        • Cd200r1 antibody
        • Cell surface glycoprotein CD200 receptor 1 antibody
        • Cell surface glycoprotein OX2 receptor 1 antibody
        • Cell surface glycoprotein OX2 receptor antibody
        • cell surface glycoprotein receptor CD200 antibody
        • CRTR 2 antibody
        • CRTR2 antibody
        • HCRTR2 antibody
        • MO2R1_HUMAN antibody
        • MOX2 receptor antibody
        • MOX2R antibody
        • OX2R antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD200R antibody [OX102] (ab34135)
        Flow Cytometry - PE Anti-CD200R antibody [OX102] (ab34135)This image is courtesy of an Anonymous Abreview.
        Overlay histogram showing Rat blood cells stained with ab34135 (red line). The sample was prepared by lysing Red Blood cells and cells were fixed with formaldehyde. The cells were then incubated with the antibody for 1 hour diluted 1/100 in PBS/1% BSA/0.01% Azide. The cell population was gated on monocytes.

        See Abreview

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab34135? Please let us know so that we can cite the reference in this datasheet.

      ab34135 has been referenced in 1 publication.

      • Noh MY  et al. Mesenchymal Stem Cells Modulate the Functional Properties of Microglia via TGF-ß Secretion. Stem Cells Transl Med 5:1538-1549 (2016). PubMed: 27400795

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Flow Cytometry abreview for Anti-CD200R antibody [OX102] (Phycoerythrin)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Monkey Cell (from whole blood)
      Specification
      from whole blood
      Preparation
      Cell harvesting/tissue preparation method: after lysis of red blood cells
      Sample buffer: PBS/BSA(1%)/azide(0.01%)
      Fixation
      Formaldehyde
      Permeabilization
      No
      Gating Strategy
      on monocytes
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 23 2011

      Flow Cytometry abreview for Anti-CD200R antibody [OX102] (Phycoerythrin)

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Rat Cell (from blood)
      Specification
      from blood
      Preparation
      Cell harvesting/tissue preparation method: after lysis of red blood cells
      Sample buffer: PBS/BSA(1%)/azide(0.01%)
      Fixation
      Formaldehyde
      Permeabilization
      No
      Gating Strategy
      on monocytes
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 23 2011

      Flow Cytometry abreview for Anti-CD200R antibody [OX102] (Phycoerythrin)

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (from blood)
      Specification
      from blood
      Preparation
      Cell harvesting/tissue preparation method: after red blood cell lysis
      Sample buffer: PBS/BSA(1%)/azide(0.01%)
      Fixation
      Formaldehyde
      Permeabilization
      No
      Gating Strategy
      on monocytes
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 23 2011

      Flow Cytometry abreview for Anti-CD200R antibody [OX102] (Phycoerythrin)

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (PBMC)
      Specification
      PBMC
      Preparation
      Cell harvesting/tissue preparation method: from buffy coat
      Sample buffer: PBS/BSA(1%)/azide(0.01%)
      Permeabilization
      No
      Gating Strategy
      monocytes
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 23 2011

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      For licensing inquiries, please contact partnerships@abcam.com

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