Key features and details
- PE Rat monoclonal [KT3] to CD3
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Product namePE Anti-CD3 antibody [KT3]
See all CD3 primary antibodies
DescriptionPE Rat monoclonal [KT3] to CD3
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityThis antibody recognises the mouse CD3 antigen which is expressed by mature T cells.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Full length native protein (purified) corresponding to Mouse CD3.
- Flow Cyt: Mouse spleen, Canine blood leucocytes, Human PBMC
Purified IgG conjugated to R. Phycoerythrin (RPE).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA, 5% Sucrose
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab22268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesContains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
ab22268 staining CD3 in mouse spleen cells by Flow Cytometry analysis.
ab22268 used in Flow Cytometry.
Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab22268 used at a 1/100 dilution for 1 hour at 4°C.
The gating strategy used was for lymphocytes.
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
Gating Strategy: Lymphocytes
ab22268 has been referenced in 1 publication.
- DeClue AE et al. Identification of immunologic and clinical characteristics that predict inflammatory response to C. Novyi-NT bacteriolytic immunotherapy. BMC Vet Res 14:119 (2018). Dog . PubMed: 29606140