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Immunology Adaptive Immunity T Cells CD
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PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)

  • Datasheet
  • SDS
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Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)

    Key features and details

    • PE Rat monoclonal [C363.29B] to CD3 epsilon
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2c

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    Overview

    • Product name

      PE Anti-CD3 epsilon antibody [C363.29B]
      See all CD3 epsilon primary antibodies
    • Description

      PE Rat monoclonal [C363.29B] to CD3 epsilon
    • Host species

      Rat
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Specificity

      ab25380 recognises CD3 epsilon.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Epitope

      The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
    • General notes

      In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.3
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 16% Sucrose

      Stabilising agent.
    • Concentration information loading...
    • Purity

      Affinity purified
    • Primary antibody notes

      In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.
    • Clonality

      Monoclonal
    • Clone number

      C363.29B
    • Isotype

      IgG2c
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Murine Lineage Negative
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Alternative Versions

      • FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
      • PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (ab25531)
    • Isotype control

      • PE Rat IgG2c, kappa monoclonal [SB68b] - Isotype control (ab154450)
    • Recombinant Protein

      • Recombinant human CD3 epsilon protein (Active) (ab167755)

    Applications

    Our Abpromise guarantee covers the use of ab25380 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1µg for 106 cells.

    Target

    • Function

      The CD3 complex mediates signal transduction.
    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P07766 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12501 Mouse
      • SwissProt: P22646 Mouse
      • Unigene: 210361 Mouse
      • Alternative names

        • CD3 epsilon antibody
        • CD3e antibody
        • CD3e antigen antibody
        • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
        • CD3E antigen epsilon polypeptide antibody
        • CD3E antigen, epsilon subunit antibody
        • CD3e molecule epsilon antibody
        • CD3e molecule, epsilon (CD3 TCR complex) antibody
        • CD3e molecule, epsilon (CD3-TCR complex) antibody
        • CD3E_HUMAN antibody
        • IMD18 antibody
        • T cell antigen receptor complex epsilon subunit of T3 antibody
        • T cell surface antigen T3/Leu 4 epsilon chain antibody
        • T cell surface glycoprotein CD3 epsilon chain antibody
        • T-cell surface antigen T3/Leu-4 epsilon chain antibody
        • T-cell surface glycoprotein CD3 epsilon chain antibody
        • T3E antibody
        • TCRE antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)
        Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)

        BALB/c lymph node cells were double stained with ab25380 and with anti-IL2RB (ab24943) at 1µg/106 cells. Small lymphocytes were gated and analyzed on a flow cytometer.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab25380? Please let us know so that we can cite the reference in this datasheet.

      ab25380 has been referenced in 1 publication.

      • Tarrant TK  et al. G protein-coupled receptor kinase-3-deficient mice exhibit WHIM syndrome features and attenuated inflammatory responses. J Leukoc Biol 94:1243-51 (2013). Mouse . PubMed: 23935208

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab25380.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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