PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)
![Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)](https://www.abcam.com/ps/products/25/ab25380/Images/ab25380-35923-anti-cd3-epsilon-antibody-c36329b-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)")
Key features and details
- PE Rat monoclonal [C363.29B] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2c
Overview
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Product name
PE Anti-CD3 epsilon antibody [C363.29B]
See all CD3 epsilon primary antibodies -
Description
PE Rat monoclonal [C363.29B] to CD3 epsilon -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD3 epsilon. IL-4 producing Th2 cell lines including D10
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Epitope
The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. -
General notes
In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. -
Clonality
Monoclonal -
Clone number
C363.29B -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
|
| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (ab25380)
BALB/c lymph node cells were double stained with ab25380 and with anti-IL2RB (ab24943) at 1µg/106 cells. Small lymphocytes were gated and analyzed on a flow cytometer.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25380 has been referenced in 1 publication.
- Tarrant TK et al. G protein-coupled receptor kinase-3-deficient mice exhibit WHIM syndrome features and attenuated inflammatory responses. J Leukoc Biol 94:1243-51 (2013). Mouse . PubMed: 23935208